- TraceMedia™ Cetrimide Agar is used for the selective cultivation and differentiation of Pseudomonas aeruginosa. Cetrimide is a quaternary ammonium compound with antibacterial activity against a range of Gram-positive organisms and some Gram-negative organisms.
- Pseudomonas aeruginosa produces several water-soluble iron-chelating agents, including yellow-green or brownish fluorescent pyoverdin. When pyoverdin combines with water-soluble blue-green pyocyanin, the characteristic bright bluish-green color of Pseudomonas aeruginosa is produced. The addition of magnesium chloride and potassium sulfate enhances the production of these iron-chelating agents.
- After incubation, observe the growth of bacteria and the presence of colonies:
- Pseudomonas aeruginosa colonies appear yellow-green or brownish and fluoresce under UV light. Presumptive identification based on colony morphology needs to be confirmed using further tests such as the oxidase test and subculture on media to detect pyoverdin and pyocyanin.
- Homogeneity: The medium is uniform in color, non-clumping, and does not separate into layers.
- Medium color: Pale greenish-blue agar, slightly opaque.
- Sterility: No bacterial/fungal growth after incubation at 36 ± 1°C for 48 – 72 hours.
- Storage: Store at 2 – 8°C.
- Transportation: Ambient temperature.
- Packaging: 10 discs/box.
- Expiration Date: 03 months from the date of production.