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Dilution and enrichment buffer
Antibiotic disc dispenser
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The product “TraceMedia™ Columbia Sheep/Chocolate Bacitracin” is a pre-poured culture medium on 90mm Petri dishes used for the cultivation and isolation of bacterial pathogens from respiratory specimens.
PURPOSE
- TraceMedia™ Columbia Sheep/Chocolate Bacitracin is a nutrient-rich dual-compartment culture medium containing Blood Agar (BA) and Chocolate Agar with Bacitracin (CAHI).
- BA: This medium is used for cultivation and differentiation of Streptococcus bacterial strains, distinguishing three types of hemolytic reactions (α, β, γ).
- CAHI: This selective medium is designed for the isolation of H. influenzae.
PRINCIPLE
TraceMedia™ Columbia Sheep/Chocolate Bacitracin
- BA Compartment: The Columbia Agar Base is supplemented with sheep blood, providing essential growth factors and enabling the determination of bacterial hemolytic reactions. On the surface of the blood agar plate, bacterial colonies with clear zones of hemolysis are classified as β-hemolytic. Colonies surrounded by a greenish zone indicate α-hemolysis. Gamma-hemolytic organisms do not produce any hemolytic reactions on the agar surface.
- CAHI Compartment: The horse blood in the chocolate agar provides essential factors X and V for the growth of H. influenzae, while the addition of Bacitracin inhibits the growth of Gram-positive bacteria.
INTERPRETATION OF RESULTS
- After incubation for the specified time, observe bacterial growth and characteristics on the surface of the culture medium.
- Further identification of isolated bacterial strains should be conducted using appropriate tests.
The relevant samples are directly inoculated onto the agar plates.
- If the agar plates have been refrigerated, allow them to warm up to room temperature before inoculating the samples.
- Inoculate the samples onto the agar plates.
- Incubate the plates in an aerobic condition at 35 – 37°C for 16 – 24 hours.
BacterLab conducts quality testing for each batch of products using standard ATCC strains. The results of the culture are as follows:
| Microorganism strain | Strain Incubation Conditions | Growth result |
| Compartment BA | ||
| Streptococcus pneumonia ATCC 49619 | 35 – 370C 5% CO2, 16–24h |
Good growth, α-hemolytic bacteria |
| Streptococcus pyogenes ATCC 19615 | Good growth, β-hemolytic bacteria | |
| Enterococcus faecalis ATCC 29212 | Good growth, non-hemolytic bacteria | |
| CAHI Compartment | ||
| Haemophilus influenzae ATCC 49766 | 35 – 370C 5% CO2, 16–24h |
Good growth |
| Streptococcus pneumonia ATCC 49619 | Weak to no growth | |
| Neisseria.meningitidis ATCC 13090 | Weak to no growth | |
In 1 liter of BA (Blood Agar) medium:
| For 1 liter of BA medium: | |
| Peptone | 10,0 g |
| Triptose | 10,0 g |
| Sodium chloride | 5,0 g |
| Sheep blood | 5-7% |
| pH of the complete medium at 25°C: 7.4 ± 0.2 | |
| For 1 liter of CAHI medium: | |
| Casein Peptone | 23,0 g |
| Meat Peptone | 7,5 g |
| Sodium chloride | 5,0 g |
| Dipotassium Phosphate | 4,0 g |
| Bacitracin | 0, 175 g |
| Horse blood | 5-7% |
| pH of the complete medium at 25°C: 7.2 ± 0.2 | |
*Please note that these compositions are for reference only. Our company can customize the components according to specific requirements or tender documents.
| Product name | Product Code | Packing |
| TraceMedia™ Columbia Sheep/Chocolate Bacitracin | TM10922 | 10 discs/box; 20 discs/box |
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