INTENDED USE
- TraceMedia™ Sheep Blood Agar is a medium used for cultivation and isolation of various microorganisms.
- It is also used to differentiate bacterial hemolysis types: alpha, beta, and gamma.
- The medium is employed for culturing and isolating infectious agents from respiratory specimens, throat swabs, nasal secretions, etc.
PRINCIPLE
- TraceMedia™ Sheep Blood Agar consists of Columbia Blood Agar Base supplemented with sheep blood, creating a medium that provides growth factors and allows for the determination of bacterial hemolytic reactions.
- On the surface of the agar plate, bacteria with a clear zone of hemolysis are classified as beta hemolysis.
- Bacteria that partially degrade hemoglobin, creating a greenish zone around the colonies, are classified as alpha hemolysis.
- Gamma hemolysis refers to no hemolysis observed on the surface.
READING THE RESULTS
- After incubation for the required time, observe the growth of colonies on the agar plate and their characteristics.
- Further identification of isolated bacteria must be conducted through appropriate tests.