BacterChrom™ Serratia Plates

Ready-to-use chromogenic medium on 90mm plates for isolating and detecting Serratia marcescens.
Code: 01022
Packaging: 10 plates/box or as per customer requirements.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 03 months from the date of manufacture.

INTENDED USE

BacterChrom™ Serratia is a selective and differential chromogenic culture medium designed for the qualitative direct detection of colonization with Serratia marcescens. It is primarily used in intensive care units (ICUs), particularly neonatal ICUs, to aid in infection prevention and control. Additionally, it can be utilized in hygiene monitoring of clinical environments through surface sampling.
The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.

PRINCIPLES

BacterChrom™ Serratia uses chromogenic substrates that react with specific enzymatic activities of target microorganisms to produce distinct colony colors. This differentiation allows for visual identification of Serratia marcescens. The selective agents incorporated into the medium inhibit the growth of non-target organisms, ensuring specificity and reducing interference from competing flora.

Allow to warm to room temperature before inoculation, dry the plates in an incubator with the covers partially removed.
Direct streaking sample onto the plate
Incubate in aerobic conditions at 35 – 37°C for 18 – 24 hours

RESULTS

Microorganism	Typical colony apprearance
Serratia marcescens	Green-blue to metallic blue
E. coli	Dark pink to reddish
Pseudomonas	Colourless +/- natural pigmentation
Morganella	Brown halo
Yeasts, Gram (+) bacteria and other Gram (-) bacteria	Inhibited

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

Reference strains	Incubation conditions	Expected results
S. marcescens ATCC 13880	Incubate for 18 – 24 hours at 35 – 37 °C	Metallic blue colonies
P. aeruginosa ATCC 9027		Colourless to yellow colonies
S. aureus ATCC 25923		Inhibited
E. faecalis ATCC 29212		Inhibited
E. coli ATCC 35218		Inhibited

For 1 liter of medium

Peptone and yeast	20,0 g
Salt	5,0 g
Chromogenic mix and selective mix	0,8 g
Agar	15,0 g
Growth factor	1,7 g
Liquid form	2 mL

pH of the ready-to-use medium at 25°C: 7,1 ± 0,2

*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.

Product Name	Code	Packaging
BacterChrom™ Serratia	01022	10 plates/box or as per customer request.