BacterTube™ Thioglycollate Broth

Ready-to-use liquid medium used for the cultivation and isolation of obligate and facultative anaerobic and microaerophilic bacteria.
Code: 08017
Packaging: 50 tubes/ box or as per customer request.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 6 months from the date of manufacture.

INTENDED USE

BacterPlate™ Thioglycollate Broth is designed for the cultivation and isolation of obligate and facultative anaerobic and microaerophilic bacteria. It is also suitable for aseptic process simulations in pharmaceutical and clinical laboratories.

PRINCIPLES

This medium contains peptone from casein and yeast extract, which provide essential nutrients for bacterial growth. D(+)-Glucose serves as an energy source. Sodium thioglycollate and L-cystine act as reducing agents, creating an anaerobic environment by lowering the oxidation-reduction potential. This environment supports the growth of anaerobes and prevents the accumulation of peroxides, which can be harmful to certain microorganisms. The sulfhydryl groups in these reducing agents can inactivate heavy metals, making the medium suitable for samples containing such compounds.

Allow the medium tube to stabilize at room temperature.
Using aseptic techniques, inoculate the medium with the sample material, ensuring the sample reaches the bottom of the bottle.
To maintain anaerobic conditions, overlay the medium with a 1 cm layer of sterile liquid paraffin or agar solution if necessary.
Incubate at 30 – 35°C for several days, depending on the growth requirements of the target organisms.

RESULTS

Microoraganisms	Signs of organism growth
Candida albicans	Flocculated Growth
Clostridium	Growth and/or opaque colonies
Peptostreptococcus (anaerobic)	Growth and/or opaque colonies
Clostridium perfringens	Growth and/or opaque colonies
Bacteroides fragilis	Growth and/or opaque colonies
Bacteroides vulgatus	Growth and/or opaque colonies
Staphylococcus aureus subsp. aureus	Growth and/or opaque colonies
Pseudomonas aeruginosa	Growth and/or opaque colonies
Micrococcus luteus	Growth and/or opaque colonies
Streptococcus pneumoniae (Pneumococcus)	Growth and/or opaque colonies
Escherichia coli	Growth and/or opaque colonies
Salmonella typhimurium	Growth and/or opaque colonies
Salmonella (general)	Growth and/or opaque colonies

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

Reference strains	Incubation conditions	Expected results
Clostridium perfringens ATCC 13124	24 – 72 hours, 35 – 37°C	Good growth
Staphylococcus aureus ATCC 25923		Good growth
Pseudomonas aeruginosa ATCC 27853		Good growth
Eschirichia coli ATCC 25922		Good growth

For 1 liter of medium

pH of the ready-to-use medium at 25°C: 7,1 ± 0,2

*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.

Product Name	Code	Packaging
BacterTube™ Thioglycollate Broth	08017	50 tubes/ box or as per customer request.