BacterTube™ Cary Blair Broth

INTENDED USE

BacterTube™ Cary Blair Broth is recommended for collection and shipment of clinical specimens.

INTENDED USE

BacterTube™ Cary Blair Broth is prepared with minimal nutrients to increase the survival of the organisms without multiplying. Sodium thioglycollate is incorporated in the medium to provide a low oxidation-reduction potential. The pH of the medium is relatively alkaline which minimizes the bacterial destruction due to the formation of acid. Phenol red is added as indicator which is red at alkaline pH, while yellow at acidic pH. Medium can maintain viability of fastidious microorganisms for only a short period of time. It is recommended that best results are obtained by direct inoculation of isolate on enriched medium at the same time specimen is inoculated into transport medium.

Ensure the medium reaches room temperature before use.
Collect clinical samples following standard protocols.
Insert or place the sample into the BacterTube™ Cary Blair.
Transport the sample to the laboratory within 24–48 hours under cool conditions.

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

Reference strain	Incubation conditions	Expected result
Salmonella typhimurium ATCC 14028	18 – 24 hours at 35 – 37⁰C	Good Growth on Subculture Medium
V.parahaemolyticus ATCC 17802		Good Growth on Subculture Medium
Shigella flexneri ATCC 12022		Good Growth on Subculture Medium
Klebsiella pneumoniae ATCC 13883		Good Growth on Subculture Medium

For 1 liter of medium

Disodium hydrogen phosphate	1,1 g
Sodium thioglycollate	1,1 g
Sodium chloride	5,0 g
Calcium chloride	0,09 g

pH of the ready-to-use medium at 25⁰C: 8,4 ± 0,2

*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.

Product Name	Code	Packaging
BacterTube™ Cary Blair Broth	08053	50 tubes/ box or as per customer request.