BacterPlate™ Sabouraud Dextrose Agar (SDA)

Enumeration of yeasts and molds in non-sterile products, according to the Pharmacopeia

• Dry the plates in an incubator with the covers partially removed.
• Inoculate with 0,1 mL of the test sample on the surface of the plates and spread around evenly with sterile triangle
• Use of membrane filters are also possible; deposit the membrane on the surface of the agar, filtered side up, after filtration of a cosmetic test product.

Cosmetics, demonstration of the efficacy of a neutralizer (NF EN ISO 11930)

• Transfer 1 mL of each trial (test sample and controls) into plates.
• Pour over roughly 15 mL of molten media maintained at 44 – 47 °C, per plate.
• Mix well and let solidify on a cold, flat surface.
• Incubate at 30 – 35 °C for 2 to 3 days for the enumeration of Candida albicans.

Note : The media can be used for the enumeration of yeasts and molds in cosmetic products known to be free of contamination by bacteria according to the standard NF EN ISO 16212. In this case, inoculate in depth, on the surface or by filtration and incubate 5 to 7 days at 20 – 25 °C

RESULTS

• Following incubation, observe the growth of yeasts and molds.
• Enumerate the plates containing less than 100 colonies or thallus.