BacterPlate™ R2A Agar

Ready-to-use medium on 90mm plates for the enumeration of microorganisms in water
Code: 05057
Packaging: 10 plates/ box or as per customer request.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 03 months from the date of manufacture.

INTENDED USE

BacterPlate™ R2A Agar is a medium destined for the enumeration of total viable aerobic microorganisms in treated water such as purified water, highly purified water and water for injectable preparation.

PRINCIPLES

Present in small quantity, the nutritive elements permit the development of stressed microorganisms. The combination of mineral salts maintains the osmotic balance. The presence of sodium pyruvate activates the bacterial metabolism.

Allow the agar plates to reach room temperature. Dry the plates in an incubator by slightly opening the lid.
Aseptically filter through a membrane a known volume of the sample to test.
Deposit the membrane on the surface of the agar, filtered side up and making sure that the membrane and agar are in close contact.
Incubate at 30 – 35 °C for at least 5 days.

INTENDED USE

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

Reference strains	Incubation conditions	Expected results
P. aeruginosa ATCC 9027	30–35°C for 48 hours	Good growth

For 1 liter of medium

Yeast extract	0,5 g
Proteose peptone	0,5 g
Acid hydrolysate of casein	0,5 g
Glucose	0,5 g
Starch	0,5 g
Dipotassium phosphate	0,3 g
Magnesium sulfate, anhydrous	0,024 g
Sodium pyruvate	0,3 g
Agar	15,0 g

pH of the ready-to-use medium at 25°C: 7,2 ± 0,2

*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.

Product Name	Code	Packaging
BacterPlate™ R2A Agar	05057	10 plates/ box or as per customer request.