BacterBlood™ Two-Phase Blood Culture Bottle

• Collect blood before antibiotics or at fever onset. Use 8 – 10 mL for adults or 1 – 3 mL for children, maintaining a blood-to-media ratio of 1:5.
• Remove the protective seal by tearing off the shrink wrap and pulling off the green cap without unscrewing the lid.
• Disinfect the rubber stopper with 70% alcohol and allow it to dry.
• Insert a sterile needle through the rubber stopper and inject the blood sample into the liquid phase.
• Replace the protective cap and gently shake to mix the blood with the liquid phase. Ensure the solid phase is moistened.
• Incubate at 35–37 °C for 5–7 days.
• Monitor for turbidity, color changes.

INTENDED USE

Microorganisms growing in broth bottles:

• Staphylococcus aureus: Typically grows as small, uniform particles with a pellicle (film) on the surface.
• Streptococci: Usually settle at the bottom or stick to the sides of the bottle in clusters resembling cotton wool or crumbs.
• Gram-negative bacilli: Generally cause uniform turbidity in the broth (except for Salmonella, which may leave the broth clear while forming a white layer above the bottom of the bottle).
• Pseudomonas spp.: Grow with a surface pellicle resembling fermented pickles, causing turbid broth, sometimes with bubbles.

Subculturing positive blood culture bottles and incubation:

• Shake the broth bottle gently.
• Use a syringe to withdraw approximately 0,5 mL of broth.
• Place one drop of the broth onto a clean glass slide, allow it to air dry, fix it, and perform a Gram stain. Make a preliminary assessment of the bacterial morphology, arrangement, and staining characteristics.
• Subculture the broth onto Blood Agar, Chocolate Agar, and MacConkey Agar. Use a sterile inoculating loop to streak evenly across the agar surface to form distinct colonies.