BacterChrom™ Enterobacteria Plates

Ready-to-use chromogenic medium on 90mm plates for the detection and enumeration of Enterobacteriaceae
Code: 01033
Packaging: 10 plates/box or as per customer requirements.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 03 months from the date of manufacture.

INTENDED USE

BacterChrom™ Enterobacteria is a selective chromogenic medium designed for the detection, enumeration, and differentiation of Enterobacteriaceae in food, environmental, and industrial samples. It is widely used in food safety testing and quality control, helping to identify contamination at various stages of the production process.
This medium is intended for laboratory use and should be operated under appropriate conditions by trained personnel. It is not suitable for clinical diagnostic use.
The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.

PRINCIPLES

BacterChrom™ Enterobacteria utilizes chromogenic substrates that react with specific enzymatic activities of Enterobacteriaceae. These reactions result in distinct colony colors that allow for differentiation among species:
- Escherichia coli produces blue colonies, with or without a blue halo.
- Other Enterobacteriaceae appear pink to red.
- Non-target Gram-positive bacteria are inhibited, while most other Gram-negative bacteria are suppressed.
The selective agents incorporated in the medium ensure specificity by suppressing non-target organisms, allowing for clear and accurate identification

Allow to warm to room temperature before inoculation, dry the plates in an incubator with the covers partially removed.
Direct streaking sample onto the plate
Incubate in aerobic conditions at 37°C for 24 hours. If searching for psychotropic Enterobacteriaceae, incubate at 30°C

RESULTS

Microorganism	Typical colony apprearance
E. coli	blue with or without blue halo
Proteus	red with swarming
Other Enterobacteriaceae	pink to red
Gram (+) bacteria	inhibited
Other Gram (-) bacteria	mostly inhibited

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

For 1 liter of medium

Peptone and yeast extract	22,0 g
Chromogenic and selective mix	0,7 g
Mineral salt	4,9 g
Agar	11,0 g
Growth factors	0,3 g

pH of the ready-to-use medium at 25°C: 7,4 ± 0,2

*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.

Product Name	Code	Packaging
BacterChrom™ Enterobacteria	01033	10 plates/box or as per customer request.