BacterContact™ Pro Chocolate + LTH

Ready-to-use medium on 60mm plates for surface microbiological sampling with the addition of a neutralizer to neutralize surface disinfectants
Code: 12022
Packaging: Three-layer packaging irradiated with 10 discs per box, or as per customer requirements.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 9 months from the date of manufacture.

INTENDED USE

BacterContact™Pro Chocolate + LTH is a culture medium for growing fastidious bacteria, used for surface microbiological sampling, supplemented with neutralizing agents to inactivate surface disinfectants.
The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.

PRINCIPLES

BacterContact™Pro Chocolate + LTH: This culture medium is suitable for the growth of fastidious bacteria such as Neisseria gonorrhoeae, Neisseria meningitidis, and Haemophilus spp. It is used for isolating infectious agents from cerebrospinal fluid or other sterile specimens. The lysis of red blood cells gives the medium a brown color and releases factor X (hemin) and factor V (nicotinamide adenine dinucleotide, NAD), which are essential for the growth of Neisseria gonorrhoeae, Neisseria meningitidis, and Haemophilus spp. Lecithin (L), Tween 80 (T), and Histidine (H) act as neutralizers for residual disinfectant chemicals.

Prepare the surface to be tested: Clean the surface to be tested using a 70% alcohol solution or another cleaning solution. Then, wait for the surface to dry completely.
Open the pre-packaged BacterContact plates: Ensure that the packaging of the plates is not torn or damaged before opening.
Place the Contact plate on the surface to be tested: Press the Rodac plate onto the surface to be tested. The recommended contact time between the plate and the test surface is 10 seconds with a pressing force of 500g.
Seal the Rodac plate: Make sure that the lid of the Rodac plate is tightly closed. Wipe the surface again with 70% alcohol.
Incubate under the following conditions
- 30 – 35 °C for 72 ± 6 hours (NF EN ISO 21149, NF EN ISO 18415)
- 20 – 25 °C for 3 to 5 days for Total Microbial Count (Pharmacopoeia

RESULTS

After incubation for the required period, typically after 72 hours, the plates are examined for the presence of microorganisms. The results are evaluated by counting the number of microbial colonies on the plates.

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

For 1 liter of medium

pH of the ready-to-use media at 25 °C: 7,3 ± 0,2

*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.

Product Name	Code	Packaging
BacterContact™ Pro Chocolate + LTH	12022	Three-layer packaging irradiated with 10 discs per box, or as per customer requirements.