BacterContact™ Pro Sabouraud Dextrose Agar + LT

Ready-to-use medium on 60mm plates for surface microbiological sampling with added neutralizers to inactivate surface disinfectants
Code: 12020
Packaging: Three-layer packaging irradiated with 10 discs per box, or as per customer requirements.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 9 months from the date of manufacture.

INTENDED USE

BacterContact™ Pro Sabouraud Dextrose Agar + LT is a fungal culture medium used for surface microbiological sampling, supplemented with neutralizing agents to inactivate surface disinfectant.
The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.

PRINCIPLES

BacterContact™ Pro Sabouraud Dextrose Agar + LT: It is a medium formulated with a pH of approximately 5,6, which promotes the growth of fungal species, particularly dermatophytes, while inhibiting the growth and development of other bacteria in the sample. The product contains peptone, which provides a source of amino acids and nitrogen for growth and development. Dextrose serves as a carbon source and is considered an energy source. Lecithin (L), Tween 80 (T) act as neutralizers for residual disinfectant chemicals.

Prepare the surface to be tested: Clean the surface to be tested using a 70% alcohol solution or another cleaning solution. Then, wait for the surface to dry completely.
Open the pre-packaged BacterContact plates: Ensure that the packaging of the plates is not torn or damaged before opening.
Place the Contact plate on the surface to be tested: Press the Rodac plate onto the surface to be tested. The recommended contact time between the plate and the test surface is 10 seconds with a pressing force of 500g.
Seal the Rodac plate: Make sure that the lid of the Rodac plate is tightly closed. Wipe the surface again with 70% alcohol.
For total yeast and mold count (Microbial Limit Test), plates should be incubated for 5 to 7 days at 20- 25 °C (EP/USP) prior to colony counting

RESULTS

After incubation for the required period, typically 3–5 days, the plates are examined for the presence of fungi. The results are evaluated by counting the number of fungal colonies on the plates.

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

Typical culture response after 48-72 hours of incubation at 20-25 °C (NF EN ISO 11133):

For 1 liter of medium

Dextrose	40,0 g
Pancreatic digest of animal tissues	5,0 g
Pancreatic digest of casein	5,0 g
Polysorbate (Tween) 80	5,0 mL
Lecithin	0,7 g
Agar	15,0 g

pH of the ready-to-use media at 25 °C: 5,6 ± 0,2

*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.

Product Name	Code	Packaging
BacterContact™ Pro Sabouraud Dextrose Agar + LT	12020	Three-layer packaging irradiated with 10 discs per box, or as per customer requirements.