BacterContact™ Pro Tryptic Soy Agar + LTHTh

Ready-to-use medium on 60mm plates for surface microbiological sampling with the addition of a neutralizer to neutralize surface disinfectants
Code: 12015
Packaging: Three-layer packaging irradiated with 10 discs per box, or as per customer requirements.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 9 months from the date of manufacture.

INTENDED USE

BacterContact™ Pro Tryptic Soy Agar + LTHTh for surface microbiological sampling with the addition of a neutralizer to neutralize surface disinfectants.
The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.

PRINCIPLES

BacterContact™ Pro Tryptic Soy Agar + LTHTh: Pancreatic Digest of Casein is a type of peptide consisting of amino acids extracted from milk protein, which facilitates bacterial growth; Peptic Digest of Soybean Meal is a peptide extracted from soybeans, providing nutrients and growth factors; Sodium Chloride creates a saline environment to maintain the pH balance inside and outside the bacteria; lecithin (L), tween 80 (T), histidine (H), and sodium thiosulfate (Th) act to neutralize residual disinfectant chemicals.

Preparation:

Prepare a test diagram for the areas that are to be tested and label plates with the corresponding location identification. Ensure that the label cannot be readily wiped off or removed. RODAC plates are prepared so that the agar surface is convex for sampling flat surfaces.
Prior to sampling, the plates should be warmed to room temperature in the plastic sleeve for approximately 15 – 20 minutes with agar up and the lid down. Remove the quantity of plates from the sleeve that are required for testing. The location/site identification should be written on the base (agar portion, not the lid) of the plate.

Sampling:

While wearing gloves, remove the lid from the plate with one hand. With the other hand, hold the base (agar portion of the plate) with thumb and middle finger. Use the index finger to gently press the plate on the test surface. Make sure the entire agar surface touches the test surface. Do not move the plate laterally while sampling as it will spread contaminants making enumeration difficult. Place the lid back on the plate and tape closed. Be sure to clean the test area after sampling to remove any residual growth media remaining on the surface.
Evaluation of Results: Incubate under the following conditions
- 30 – 35 °C for 72 ± 6 hours (NF EN ISO 21149, NF EN ISO 18415)
- 20 – 25 °C for 3 to 5 days for Total Microbial Count (Pharmacopoeia

RESULTS

After incubation, check the plates to see if microorganisms have appeared. The results will be evaluated by counting the number of microorganisms on the plates.

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

For 1 liter of medium

Tryptone	15,0 g
Papaic digest of soybean meal	5,0 g
Sodium chloride	5,0 g
Polysorbate (Tween) 80	5,0 mL
Lecithin	0,7 g
Histidine	0,5 g
Natri thiosulfate	0,05 g
Agar	15,0 g

pH of the ready-to-use media at 25 °C: 7,3 ± 0,2

*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.

Product Name	Code	Packaging
BacterContact™ Pro Tryptic Soy Agar + LTHTh	12015	Three-layer packaging irradiated with 10 discs per box, or as per customer requirements.