BacterPlate™ Bromocresol Purple Agar (BCP)

  • Ready-to-use medium on 90mm plates for differentiating between Enterobacteriaceae and Coliforms
  • Code: 05032
  • Packaging: 10 plates/ box or as per customer request.
  • Storage Conditions: 2–8°C.
  • Transportation: Ambient temperature.
  • Shelf Life: 03 months from the date of manufacture.

INTENDED USE

  • BacterPlate™ Bromocresol Purple Agar (BCP) is a non-selective medium, used for the differentiation of Enterobacteriaceae. It is used for the confirmation of coliforms in the context of the standard T90 – 425, for the bacteriological examination of containers and recipients used in water bottling.

PRINCIPLES

  • Fermentation of lactose to acid is revealed in the presence of bromocresol purple by a color change from violet-blue to yellow.
  • Lactose-negative bacteria form blue colonies.
  • Dry the plates in an incubator with the covers partially removed.
  • Inoculate by streaking on the surface.
  • Incubate at 37°C for 18 to 24 hours.

NOTE: A longer incubation time than 24 hours will lead to a re-alkalinization of the media and could modify the color of the colonies, leading to errors.

RESULTS

  • Lactose-positive colonies are yellow and are differentiated as a function of the following characteristics :
    • mucoid colonies : Klebsiella, Escherichia coli.
    • colonies bluish at the edges: slow lactose-fermenting Escherichia coli.
    • smooth or rough colonies which are transparent when examined with back lighting : Escherichia coli, Citrobacter.
  • Lactose – negative colonies are blue

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

Reference strains Incubation conditions Standard
E. coli ATCC 35218 35 – 37°C

18 – 24 hours

 Good growth
S. Enteritidis ATCC 13076 Good growth
P. aeruginosa ATCC 9027 Good growth

For 1 liter of medium

Tryptone 5,0 g
Meat extract 3,0 g
Lactose 10,0 g
Bromocresol purple 25,0 mg
Agar 13,0 g

pH of the ready-to-use medium at 25°C: 7,0 ± 0,2

*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.

Product Name Code Packaging
BacterPlate™ Bromocresol Purple Agar (BCP) 05032 10 plates/ box or as per customer request.

USER MANUAL_BacterPlate™ Bromocresol Purple Agar (BCP)
MSDS_BacterPlate™ Bromocresol Purple Agar (BCP)
COA_BacterPlate™ Bromocresol Purple Agar (BCP)

Related products

BacterPlate™ Bacillus Cereus Agar

BacterPlate™ Dnase Agar

BacterPlate™ Bile Esculin Agar (BEA)

BacterPlate™ Malt Extract Agar

BacterPlate™ Columbia Agar (CBA)

BacterPlate™ Baird Parker Agar (BPA)

BacterPlate™ Hektoen Enteric Agar (HE)

BacterPlate™ R2A Agar

BacterPlate™ SDA with Chloramphenicol

BacterPlate™ Tryptic Soy Agar (TSA)