BacterPlate™ Chocolate Agar with Neomycin

Ready – to – use medium for culturing fastidious microorganisms, particularly Neisseria and Haemophilus.
Code: 05016
Packaging: 10 plates/ box or as per customer request.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 03 months from the date of manufacture.

INTENDED USE

BacterPlate™ Chocolate Agar with Neomycin is a ready-to-use selective medium designed for the isolation and cultivation of fastidious Gram-negative bacteria, particularly Haemophilus and Neisseria species. The addition of gentamicin enhances selectivity by inhibiting the growth of contaminating bacteria, making it ideal for diagnostic workflows in clinical microbiology laboratories.
The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.

PRINCIPLES

BacterPlate™ Chocolate Agar with Neomycin is formulated with:
- Defibrinated Blood: Heat-treated to release growth factors (hemin and NAD), essential for the growth of Haemophilus and Neisseria
- Neomycin: Functions as a selective agent to suppress the growth of Gram-positive bacteria.
- Enriched Nutrients: Supports the robust growth of target organisms.
This combination promotes the selective recovery and reliable differentiation of fastidious pathogens, ensuring efficient and accurate diagnostic results.

Allow the agar plates to reach room temperature. Dry the plates in an incubator by slightly opening the lid.
Inoculate in order to obtain isolated colonies.
Incubate at 35 – 37 °C, 5 – 10% CO₂ for 18 – 24 hours in optimal conditions for the culture of the inoculated germs.

RESULTS

After incubation for the required duration, observe the colonies growing on the surface of the plate for characteristic traits such as colony size, color, and morphology.
- Haemophilus influenzae: Smooth, greyish, translucent colonies.
- Neisseria gonorrhoeae: Small, shiny, grey colonies with a mucoid appearance.
Identification of the isolated bacteria must be carried out through appropriate subsequent tests

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

For 1 liter of medium

pH of the ready-to-use medium at 25⁰C: 7,3 ± 0,2

*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.

Product Name	Code	Packaging
BacterPlate™ Chocolate Agar with Neomycin	05016	10 plates/ box or as per customer request.