BacterPlate™ Salmonella Shigella Agar (SS)

Ready-to-use medium on 90mm plates for the selective culture of Salmonella and Shigella. Applied for culturing stool samples.
Code: 05019
Packaging: 10 plates/ box or as per customer request.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 03 months from the date of manufacture.

INTENDED USE

BacterPlate™ Salmonella Shigella Agar (SS) is used for the isolation of Salmonella and Shigella in fecal material. It can also be used as a second media of isolation in the context of standardized methods for the detection of Salmonella.
The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.

PRINCIPLE

BacterPlate™ Salmonella Shigella Agar (SS) is a moderately selective medium in which Gram-positive bacteria are inhibited by bile salts, brilliant green and sodium citrate. The high concentrations of citrate and sodium thiosulfate limit the development of coliform bacteria and prevent the invasion of the medium by Proteus. Fermentation of lactose to acid is revealed by the formation of red colonies in the presence of neutral red. Lactosenegative bacteria form colorless colonies. In the presence of thiosulfate and ferric citrate, hydrogen sulfide producers form colonies with a black center.

RESULTS

Salmonella species not fermenting lactose form colorless, transparent colonies, with or without a black center (H2S production).
Shigella are colorless.
Colonies of coliform bacteria are red or pink.

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

Standard Microorganism Strain	Incubation Conditions	Expected result
S. typhimurium ATCC 14028	18 – 24 hours at 35 – 37 ^oC	Good growth, colorless colonies with black centers
S. flexneri ATCC 12022		Good growth, colorless colonies
E. coli ATCC 25922		Partial inhibition, pink colonies
S. aureus ATCC 25923		Inhibited, no growth

For 1 liter of medium

pH of the ready-to-use medium at 25⁰C: 7,0 ± 0,2

*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.

Product Name	Code	Packaging
BacterPlate™ Salmonella Shigella Agar (SS)	05019	10 plates/ box or as per customer request.