BacterStain™ Crystal Violet

INTENDED USE

BacterStrain™ Crystal Violet is primarily used in the Gram staining method to differentiate between Gram-positive and Gram-negative bacteria. It is also used in cell staining, histological research, and enzyme activity determination.

PRINCIPLES

BacterStrain™ Crystal Violet is a cationic dye that binds to the bacterial cell wall, particularly to the polysaccharides and peptidoglycan in Gram-positive bacteria. During staining, Gram-positive bacteria retain the purple color due to their strong cell wall structure, while Gram-negative bacteria lose the color and are subsequently counterstained with safranin.

Used in Gram Strain Kit

Prepare a thin smear on clear, dry glass slide.
Allow it to air dry and fix by gentle heat
Flood with BacterStain™ Crystal Violet (Code: 04007) for 1 minute.
Wash with tap water
Flood the smear with BacterStain™ Lugol (Code: 04005). Allow it to remain for 1 minute
Decolourize with BacterStain™ Alcohol (Code: 04011) until the blue dye no longer flows from the smear
Wash with tap water
Counter stain with BacterStain™ Safranine (Code: 04008) for 1 minutes and rinses off with water
Allow the slide to air dry or blot dry between sheets of clean bibulous paper and examine under oil immersion objective

RESULTS

Gram-positive bacteria stain dark violet with gentian: Staphylococcus, Streptococcus, Pneumococcus, Diphtheria bacillus, Bacillus anthracis.
Gram-negative bacteria do not stain violet with gentian, so they appear pink-red with fuchsin: Neisseria gonorrhoeae, Neisseria meningitidis, Escherichia coli, Shigella, Salmonella, Vibrio cholerae.

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

For 1 liter of strain

*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.

Product Name	Code	Packaging
BacterStrain™ Crystal Violet	04007	100ml/bottle or according to the customer’s request..