BacterStain™ Lugol

A reagent is recommended for use in qualitative procedures to differentiate gram-negative from gram-positive organisms
Code: 04005
Packaging: 100ml/bottle or according to the customer’s request..
Storage Conditions: 18 – 25°C.
Transportation: Ambient temperature.
Shelf Life: 24 months from the date of manufacture.

INTENDED USE

BacterStrain™ Lugol is an iodine solution used in the Gram staining method to form a complex with Crystal Violet, helping to retain the purple color in Gram-positive bacteria. It is also used in microbiological research, to test the precipitation of substances, and as an antiseptic in medical applications.

PRINCIPLES

BacterStrain™ Lugol contains iodine and potassium iodide, which, when in contact with Crystal Violet, form a complex with the dye, helping it bind tightly to the cell wall of Gram-positive bacteria. This complex is resistant to decolorization by acid alcohol, allowing Gram-positive bacteria to retain the purple color while Gram-negative bacteria do not.

Used in Gram Strain Kit

Prepare a thin smear on clear, dry glass slide.
Allow it to air dry and fix by gentle heat
Flood with BacterStain™ Crystal Violet (Code: 04007) for 1 minute.
Wash with tap water
Flood the smear with BacterStain™ Lugol (Code: 04005). Allow it to remain for 1 minute
Decolourize with BacterStain™ Alcohol (Code: 04011) until the blue dye no longer flows from the smear
Wash with tap water
Counter stain with BacterStain™ Safranine (Code: 04008) for 1 minutes and rinses off with water
Allow the slide to air dry or blot dry between sheets of clean bibulous paper and examine under oil immersion objective

RESULTS

Gram-positive bacteria stain dark violet with gentian: Staphylococcus, Streptococcus, Pneumococcus, Diphtheria bacillus, Bacillus anthracis.
Gram-negative bacteria do not stain violet with gentian, so they appear pink-red with fuchsin: Neisseria gonorrhoeae, Neisseria meningitidis, Escherichia coli, Shigella, Salmonella, Vibrio cholerae.

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

For 1 liter of strain

Iodine	3 g
KI	6 g

*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.

Product Name	Code	Packaging
BacterStrain™ Lugol	04005	100ml/bottle or according to the customer’s request..