BacterTest™ α-Naphthol

This reagent and KOH reagent are used in MR-VP culture medium to perform VP test.
Code: 06005
Packaging: 100mL/ dropper bottle or as per customer request.
Storage Conditions: 8 – 25°C.
Transportation: Ambient temperature.
Shelf Life: 12 months from the date of manufacture.

INTENDED USE

BacterTest™ α-Naphthol is a reagent commonly used in chemical and analytical reactions to detect, identify, or quantify certain substances or ions in a sample. In the Voges-Proskauer method, α-Naphthol is one of the two main reagents (along with KOH), and its role is to aid in detecting the presence of acetoin in the culture medium.

PRINCIPLES

BacterTest™ α-Naphthol is one of the two main reagents in this test, with an important role in the detection of acetoin.
- Acetoin (or 3-hydroxybutanone) is produced during the fermentation of glucose (or other carbohydrates) by some species of bacteria.
- When the culture medium contains acetoin, the α-Naphthol reagent will react with acetoin, forming a red or pink complex.
- KOH is necessary to increase the alkalinity of the medium, helping acetoin to convert into substances that can react with α-Naphthol, creating a recognizable color.

Bacteria are cultured in prepared MR-VP medium after 24 – 48 hours at 37°C (to allow bacteria time to ferment glucose in the medium)
Adding 0,5 mL of 40% KOH solution to the sample.
Then, adding 3 – 4 drops of α-Naphthol reagent to the cultured bacteria sample.
Reacting within minutes to hours.
Observing the color change of the medium, reading and recording the results

RESULTS

Observe the color change of the environment:
- Red or pink = Positive (Bacteria that produce acetoin)
- Colorless or Yellow = Negative (Bacteria do not produce acetoin).

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

Refer to the components.

α-Naphthol	5,0 g
Ethanol (95%)	100mL

*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.

Product Name	Code	Packaging
BacterStrain™ α-Naphthol	06005	100mL/ dropper bottle or as per customer request.