BacterTube™ BHI 20% Glycerol

The medium contained in a screw-cap tube with 1 mL used for preserving bacteria and fungi.
Code: 08012
Packaging: 50 tubes/ box or as per customer request.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 6 months from the date of manufacture.

INTENDED USE

BacterTube™ BHI 20% Glycerol is used for the preservation of organisms by freezing. It is recommended for long-term storage and ensures the viability of microorganisms under low-temperature conditions

PRINCIPLES

The Brain Heart Infusion (BHI) medium provides essential nutrients required for the survival of microorganisms. A 20% concentration of glycerol is added to the broths to maintain viability of microorganisms and to protect cell membranes. Glycerol acts as a cryoprotective agent and allows for long-term bacterial preservation

Open the screw-cap tube containing the BHI 20% glycerol in in a sterile environment.
Use a sterile swab to collect bacteria or fungi from the surface of an agar plate or add the microbial suspension to the tube (not exceeding the total capacity)
Mix gently to ensure even distribution
Freeze the tubes at temperatures appropriate for your laboratory storage (-20°C or -80°C is recommended).

Note: Ensure the tube is tightly closed to prevent contamination or evaporation during storage

RESULTS

Growth of frozen stock culture on solid media indicates the presence of a viable organism

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

Product Name	Code	Packaging
BacterTube™ BHI 20% Glycerol	08012	50 tubes/ box or as per customer request.