BacterTube™ BHI Broth

A nutrient-rich medium that provides essential substances for microbial growth, suitable for culturing various types of microorganisms.
Code: 08009
Packaging: 50 tubes/ box or as per customer request.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 6 months from the date of manufacture.

INTENDED USE

BacterTube™ BHI Broth is a buffered nutrient medium designed for the culture of aerobic and anaerobic microorganisms, including yeasts and molds. It is also used for the detection of staphylocoagulase and complies with NF EN ISO 6888-1, NF EN ISO 6888-3, and NF V08-057-1 standards in food microbiology.

PRINCIPLES

BacterTube™ BHI Broth derived from the Rosenow formula (1919), contains:
- Pork brain-heart infusion: Rich in nutritive compounds to support fastidious bacterial growth.
- Gelatin digest: Supplies amino acids and peptides.
- Sodium chloride: Maintains osmotic balance.
- Disodium phosphate: Buffers pH stability.
- Glucose: Provides an energy source to enhance growth

Confirmation of Coagulase-Positive Staphylococci (NF EN ISO 6888-1 & 3):

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

Reference strains	Incubation conditions	Expected results
Staphylococcus aureus ATCC 25923	24 hours, 36°C	Growth causing turbidity in the culture medium
Escherichia coli ATCC 25922	24 hours, 36°C	Growth causing turbidity in the culture medium

For 1 liter of medium

pH of the ready-to-use medium at 25°C: 7,4 ± 0,2

*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.

Product Name	Code	Packaging
BacterTube™ BHI Broth	08009	50 tubes/ box or as per customer request.