BacterTube™ Malonate Broth

The test medium is used to differentiate Enterobacter and Escherichia based on malonate utilization.
Code: 08035
Packaging: 50 tubes/ box or as per customer request.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 6 months from the date of manufacture.

INTENDED USE

BacterTube™ Malonate Broth h is recommended for use in the differentiation of Enterobacteriaceae on the basis of malonate utilization.

PRINCIPLES

The medium distinguishes malonate-positive organisms (e.g., Klebsiella pneumoniae) from malonate-negative organisms (e.g., Escherichia coli).
- Malonate and Ammonium Sulfate: Organisms that utilize both produce sodium hydroxide, causing alkalinity and a color change to light blue or Prussian blue.
- Bromothymol Blue: pH indicator; turns blue in alkaline conditions and yellow in acidic environments due to glucose fermentation.
- Yeast Extract and Dextrose: Provide minimal growth for organisms unable to utilize malonate

Prior to inoculation, allow medium to equilibrate to room temperature
Using a light inoculum from an 18 – 24 hour pure culture, inoculate the tube.
Incubate the tube with loosened caps at 35ºC in an aerobic atmosphere for 24 – 48 hours
Observe for alkalization (blue color) at 24 and 48 hours

RESULTS

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

Reference strains	Incubation conditions	Expected results
Klebsiella pneumoniae ATCC 13883	24 – 48 hours, 35 – 37^oC	Positive: Good growth, blue color
Escherichia coli ATCC 25992	24 – 48 hours, 35 – 37^oC	Negative: Inhibited, light green color

For 1 liter of medium

pH of the ready-to-use medium at 25°C: 6,7 ± 0,2

*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.

Product Name	Code	Packaging
BacterTube™ Malonate Broth	08035	50 tubes/ box or as per customer request.