BacterTube™ Phenylalanine Deaminase Agar (PAD)

INTENDED USE

BacterTube™ Phenylalanine Deaminase Agar (PAD) is recommended for differentiating Proteus and Providencia species from other members of the Enterobacteriaceae family based on their ability to deaminate phenylalanine to form phenylpyruvic acid.

PRINCIPLES

Proteus and Providencia species can deaminate phenylalanine to produce phenylpyruvic acid.
Meat extract provides essential growth nutrients.
Sodium chloride maintains osmotic balance.
After incubation, phenylpyruvic acid reacts with ferric chloride to produce a green color, indicating a positive result

Inoculate the BacterTube™ Phenylalanine Deaminase Agar (PAD) with the cultured bacteria.
Incubate the inoculated tubes at 35 – 37°C for 24 hours.
After incubation, add the reagent to the tubes.

RESULTS

Positive result: The medium turns dark green. This color change is due to degradation products produced by phenylalanine. These products react with the iron compounds in the acidic environment, resulting in the dark green color.
Negative result: The color of the medium remains unchanged, staying straw yellow (no PPA to react with FeCl₃)

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

Reference strains	Incubation conditions	Expected results
Proteus mirabilis ATCC 12453	24 – 48 hours, 35 – 37^oC	Color change after adding 10% FeCl₃ solution: Positive result.
Escherichia coli ATCC 25922	24 – 48 hours, 35 – 37^oC	No color change after adding 10% FeCl₃ solution: Negative result

For 1 liter of medium

pH of the ready-to-use medium at 25°C: 7,3 ± 0,2

*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.

Product Name	Code	Packaging
BacterTube™ Phenylalanine Deaminase Agar (PAD)	08037	50 tubes/ box or as per customer request.