BacterTube™ Sabouraud Dextrose Broth (SDB)

INTENDED USE

BacterTube™ Sabouraud Dextrose Broth (SDB) is recommended for the detection of Candida albicans in non-sterile products according to the Pharmacopoeia. It is also used as a nutrient medium for the growth of yeasts and molds. The typical composition meets the requirements of the European and United States Pharmacopoeia.

PRINCIPLES

BacterTube™ Sabouraud Dextrose Broth (SDB) with ingredients such as meat extract and casein, which provide nitrogen and carbohydrates, it serves as a factor for the growth of yeasts, molds, and acidophilic bacteria. Glucose is the energy source.

According to the Pharmacopoeia, transfer 1 mL of the sample for testing and its serial dilutions into 10 mL of Sabouraud Dextrose Broth.
Incubate at 30 – 35°C for 3 to 5 days.
Subculture onto Sabouraud Dextrose Agar (SDA) and incubate for 24 to 48 hours at 30 – 35°C.

Note:

RESULTS

Observe the results by the growth indicated by the turbidity of the inoculated medium.

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

Reference strains	Incubation conditions	Expected results
Candida albicans ATCC 10231	72 hours, 30 – 35^oC, Aerobic	Good growth
Saccharomyces cerevisiae ATCC 9763	48 – 72 hours, 20 – 25^oC, Aerobic	Good growth
Aspergillus brasiliensis ATCC 16404	48 – 72 hours, 20 – 25^oC, Aerobic	Good growth

For 1 liter of medium

pH of the ready-to-use medium at 25°C: 5,6 ± 0,2

*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.

Product Name	Code	Packaging
BacterTube™ Sabouraud Dextrose Broth (SDB)	08051	50 tubes/ box or as per customer request.