BacterTube™ Selectine Cysteine

INTENDED USE

BacterTube™ Selectine Cysteine is recommended as a selective enrichment medium for isolating Salmonella and possibly Shigella sonnei from samples such as feces, urine, water, and food.

PRINCIPLES

The medium contains selenite, which inhibits the growth of competing bacteria while allowing the selective enrichment of Salmonella.
Tryptone provides essential nutrients, including nitrogen and vitamins.
Lactose helps maintain pH stability.
L-Cystine enhances the recovery of Salmonella.
After enrichment, the broth is subcultured on differential agar media for further isolation

BacterTube™ Selectine Cysteine can be used with the following specimens: faeces, urine, water samples and foods
Allow tubes to reach room temperature before inoculation
Add 1g of stool sample or 1ml of stool suspension into the tube. The swab sample can be directly placed into the tube containing the medium.
Completely dissolve the mixture in the tube.
Incubate under aerobic conditions for 12 – 24 hours at 35 – 37°C.
After incubation, transfer 1 – 2 drops from the tube onto selective media such as MacConkey Agar or Hektoen Enteric Agar to isolate colonies.
Continue incubating at 35–37°C.
Escherichia coli and Proteus species are not inhibited indefinitely. If the initial concentration of these microorganisms is high, it is best to subculture onto solid media after 6 hours of incubation in selenite broth.

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

Reference strains

Incubation conditions

Expected results

Salmonella typhimurium ATCC 14028

18 – 24 hours, 35 – 37°C, 5 – 10 % CO₂

Good growth

Escherichia coli ATCC 25922

Then subculture onto MacConkey

Partially or fully inhibited.

For 1 liter of medium

pH of the ready-to-use medium at 25°C: 7,0 ± 0,2

*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.

Product Name	Code	Packaging
BacterTube™ Selectine Cysteine	08005	50 tubes/ box or as per customer request.