INTENDED USE

BacterTube™ Sterile Physiological Saline (NS 0,85%) is recommended for procedures that require the use of an isotonic diluent. It is also recommended foruse in preparing test suspensions of organisms

PRINCIPLES

Saline is useful as a diluent to maintain cell integrity andviability because it lacks properties that may interfere with biochemical reactions and/or antibiotic susceptibility tests. The concentration of sodium chloride in 0.85% (normal) Saline provides osmotic protection for microbial cells. Normal saline is used for preparing microbial suspensions when it is necessary to deliver a setnumber of microbes to an identification test battery, to antimicrobial agents, or to growth media used for disk susceptibility testing. It is also used in the preparing of stock solutions and serial dilutions of antimicrobial agents. The Clinical and Laboratory Standards Institute (CLSI – formerly NCCLS) recommends the use of 0.85% Saline to adjustthe turbidity of bacterial suspensions to help maintain cell integrityand viability.

Solutions for inoculation should be prepared by inoculating the diluent with one to five isolated colonies of test organism.
The suspension should be adjusted to the concentration of the appropriate McFarland inoculum standard.

Criteria	Incubation conditions	Expected results
Sterility	18 – 24 hours, 35 – 37°C	Free from microbial contamination

For 1 liter of medium

Sodium chloride

8,5 g

pH of the ready-to-use medium at 25°C: 5,5 ± 0,2

*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.

Product Name	Code	Packaging
BacterTube™ Sterile Physiological Saline (NS 0,85%)	08019	50 tubes/ box or as per customer request.

BacterTube™ Sterile Physiological Saline (NS 0,85%)