TraceMedia™ ESBL/ MRSA

To apply for screening to detect ESBL (Extended Spectrum β-Lactamase) and MRSA (Methicillin-resistant S. aureus) bacteria, you can use the following product:
  • Product name: TraceMedia™ ESBL/MRSA Screening Agar.
  • Product code: TM11322.
  • Packaging: 10 Petri dishes per box; 20 Petri dishes per box.
  • Storage: Store at 2 – 8°C.
  • Transport: Transport at room temperature.
  • Expiration Date: 03 months from the date of manufacture.
  • The pre-poured media on 90mm Petri dishes is used to test the susceptibility of antimicrobials or the paper strip MIC on challenging-to-grow microorganisms such as Neisseria, Haemophilus, and Helicobacter pylori.
PURPOSE
  • TraceMedia™ MRSA/ESBL is a chromogenic media used to detect and differentiate MRSA (Methicillin Resistant Staphylococcus Aureus) and ESBL-producing bacteria (Extended-spectrum β-lactamase).
PRINCIPLE
TraceMedia™ MRSA/ESBL:
  • MRSA Detection: It directly detects and identifies MRSA by combining color-indicating substrates and cefoxitin. MRSA bacteria will grow in the presence of cefoxitin and produce pink to pale purple colonies due to the hydrolysis of the chromogenic substrate. Selective agents prevent the growth of gram-negative organisms, fungi, and some other gram-positive cocci.
  • ESBL Detection: This media detects Gram-negative bacteria that produce beta-lactamase enzymes that are resistant to penicillins, third-generation extended-spectrum cephalosporins (C3G), and monobactams. The ESBL agar contains a peptone mix as a nutrient source for bacterial growth. Chromogenic mix and selective mix aid in selection and produce coloration for ESBL-producing bacteria.
READING THE RESULTS
  • After incubating for the required time, usually 16-24 hours, observe the bacterial colonies growing on the surface of the plate.
  • Further identification of isolated bacteria must be carried out through appropriate tests.

The relevant samples are directly inoculated onto the agar plates.

  1. If the agar plates have been refrigerated, allow them to warm up to room temperature before inoculating the samples.
  2. Inoculate the samples onto the agar plates.
  3. Incubate the plates in an aerobic condition at 35 – 37°C for 16 – 24 hours.

BacterLab conducts quality control testing for each batch of its products using standard ATCC strains:

Microorganism strain Strain incubation conditions Growth result 
MRSA Compartment 
Staphylococcus.aureus ATCC 43300

35 – 370C, 16-24h

 Good growth, colonies appear as pale pink
Staphylococcus aureus ATCC 25923 Good growth, colonies appear as pink.
Escherichia coli ATCC 25922 Weak growth or no growth observed.
ESBL Compartment 
Klebsiella.pneumoniae ATCC 700603

35 – 370C, 16-24h

  Good growth, colonies appear as creamy white.
Escherichia coli ATCC 25922 Good growth, colonies show white hyphal structures with black spores (conidia).

The reference compositions for 1 liter of MRSA and ESBL media are as follows:

MRSA Medium:
Chromopeptone.. 35,0 g
Sodium chloride 17,5 g
Inhibitory Agents 7,52 g
Chromogen Mix 0,5 g
Cefoxitin 5,2 mg
Agar 14,0 g
pH of the complete medium at 25°C: 7.2 ± 0.2
ESBL Medium:
Agar 15,0 g
Peptone and yeast extract 17,0 g
Chromogenic mix 1,0 g
Selective mix 0,57 g
pH of the complete medium at 25°C: 7.0 ± 0.2

*Please note that this composition is for reference purposes. Additionally, Our company will prepare the components as required or according to the tender documents.

Product name Product code Packing
TraceMedia™ ESBL/ MRSA TM11322 10 plates/box; 20 plates/box

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