TraceMedia™ Hektoen Enteric Agar

  • Shigella and Salmonella Isolation Agar from Gastrointestinal Specimens.
  • Product Code: TM1822.
  • Pack Size: 10 Discs/Box, 20 Discs/Box.
  • Storage: 2 – 8°C.
  • Transportation: Ambient temperature.
  • Expiration Date: 03 months from the date of manufacture.
INTENDED USE
  • TraceMedia™ Hektoen Enteric Agar is a pre-poured agar medium in a 90mm petri dish used for selection and differentiation of pathogenic enteric bacteria from gram-negative enteric bacterial specimens.

PRINCIPLE

  • TraceMedia™ Hektoen Enteric Agar contains a high peptone content to reduce the inhibitory effect of bile salts on Shigella strains.
  • Additional carbohydrates (sucrose and salicin) provide better differentiation than lactose alone, and the low toxicity of the two indicator agents (acid fuchsin, bromothymol blue) enhances bacterial colony formation.
  • Increased lactose content helps early detection of bacteria with slow lactose fermentation. Thiosulphate and Ferric citrate are included to detect bacteria that produce H2S.

RESULT

  • After incubation for the required time, typically 18-24 hours, bacterial colonies are observed on the surface of the agar plate.
  • Further identification of isolated bacteria must be conducted using appropriate tests.
The following plates are directly streaked:
  1. If the agar plates have been refrigerated, allow them to warm up to room temperature before streaking.
  2. Inoculate the samples onto the agar plates.
  3. Incubate at aerobic conditions at a temperature of 35 – 37°C for 16 – 24 hours.

BacterLab checks the quality of each batch of products using ATCC standard strains:

Standard Strain Incubation Conditions Results
Time Temperature aerobic Conditions
Salmonella typhimurium ATCC® 14028 18h – 24h 35oC Aerobic Good growth, blue colonies with black
Shigella flexneri ATCC® 12022 18h – 24h 35oC Aerobic Good growth, green colonies
Escherichia coli ATCC® 25922 18h – 24h 35oC Aerobic Inhibited or no growth
Enterococcus faecalis ATCC® 29212 18h – 24h 35oC Aerobic No growth

TIngredients per 1 liter (Reference):

Proteose peptone 12,0 g
Yeast extract 3,0 g
Lactose 12,0 g
Sucrose 12,0 g
Salicin 2,0 g
Bile salts 9,0 g
Sodium chloride 5,0 g
Sodium thiosulfate 5,0 g
Ferric ammonium citrate 1,0 g
Bromothymol blue 65 mg
Acid fuchsin 0,1 g
Agar 14 g
The pH of the complete medium at 25°C is 7.3 ± 0.2.

*Please note that this composition is for reference purposes. Additionally, Our company will prepare the components as required or according to the tender documents.

Product name Product code Packaging
TraceMedia™ Hektoen Enteric Agar TM1822 10 Discs/Box; 20 Discs/Box

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