TraceMedia™ Mueller Hinton Agar

  • Product Code: TM3122.
  • Package: 10 Petri dishes per box.
  • Storage: Store at 2 – 8°C.
  • Transportation: Ambient temperature.
  • Expiration Date: 3 months from the manufacturing date.
PURPOSE
  • Mueller Hinton Agar is used to test the antibiotic susceptibility of fast-growing bacteria.
PRINCIPLES
  • TraceMedia™ Mueller Hinton Agar contains nutrient-rich components such as beef extract and acid hydrolysate of casein, providing amino acids, nitrogenous compounds, vitamins, and essential minerals for microbial growth.
  • Starch acts as a protective colloid against toxic substances in the environment. Additionally, the medium contains a small amount of thymidine and thymine, which can inhibit the effect of sulfonamides and trimethoprim.
  • The presence of divalent cations in the agar controls the activities of aminoglycosides, tetracycline, and colistin, especially for evaluating Pseudomonas aeruginosa.

RESULTS

  • After incubation for the required time, observe bacterial growth on the surface of the plate and the zones of inhibition around antibiotic discs. The identification of isolated bacteria must be further confirmed using appropriate tests. If isolated colonies still appear, retesting is necessary.
  • To measure the complete inhibition zones (including the antibiotic discs), invert the plate and hold it up to the light. Use a ruler graduated to millimeters to measure the diameter of the complete inhibition zones.
  • The edge of the inhibition zone should be read from the area where bacterial growth is not visible to the naked eye. Faint or small colonies at the edge of the growth inhibition zone may only be visible under magnification and should be ignored.
  • For interpreting the zone sizes, refer to CLSI M100 guidelines for each bacterial strain.

The relevant samples are directly inoculated onto the agar plates.

  1. If the agar plates have been refrigerated, allow them to warm up to room temperature before inoculating the samples.
  2. Inoculate the samples onto the agar plates.
  3. Incubate the plates in an aerobic condition at 35 – 37°C for 16 – 24 hours.

BacterLab conducts quality testing of each batch of products using the standard strains provided by the American Type Culture Collection (ATCC). The expected results for the testing are as follows:

Standard Strain incubation conditions Cultivation result 
Enterococcus faecalis ATCC 33186 16-24 hours at 35-37°C Good growth, with inhibition zone sizes within the range specified by the Clinical and Laboratory Standards Institute (CLSI)
Escherichia coli ATCC 25922 16-24 hours at 35-37°C Good growth, with inhibition zone sizes within the range specified by the Clinical and Laboratory Standards Institute (CLSI)

The components per 1 liter (reference):

Acid Digest of Casein 17.5g
Beef Extract 2g
Starch 1.5g
Sodium chloride 5g
Agar 15g
pH of the complete medium at 25°C: 7.3 ± 0.2

*Note: This composition is for reference purposes only. In addition, our company will prepare the components according to specific requirements or tender documents.

Product name: Product code Packing
TraceMedia™ Mueller Hinton Agar TM3122 10 Dishes/box

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