TraceMedia™ Mueller Hinton Agar + NaCl

  • Product code: TM3222.
  • Package: 10 dishes/box, 20 dishes/box.
  • Storage: Store at 2 – 8°C.
  • Transportation: Ambient temperature.
  • Expiration Date: 3 months from the manufacturing date.
PURPOSE:
  • TraceMedia™ Mueller Hinton Agar + NaCl is designed for testing the susceptibility of Staphylococcus aureus to the antibiotic Oxacillin through disk diffusion or MIC strip testing.

PRINCIPLE:

  • TraceMedia™ Mueller Hinton Agar + NaCl contains a nutrient-rich formula, including beef extract and acid hydrolysate of casein, providing amino acids, nitrogen compounds, vitamins, and essential minerals for microbial growth.
  • The presence of starch acts as a protective colloid to counteract the effect of toxic substances in the environment. Additionally, the medium contains a small amount of thymidine and thymine, which can inhibit the action of sulfonamides and trimethoprim.
  • The controlled concentration of divalent cations in the agar ensures standardization for the activities of aminoglycosides, tetracyclines, and colistin when evaluating Pseudomonas aeruginosa.
  • The formula is supplemented with sodium chloride, suitable for detecting Methicillin resistance in Staphylococcus aureus.
READING THE RESULTS:
  • After the required incubation period, observe bacterial growth on the surface of the agar plate and the zones of inhibition around antibiotic disks. Further identification of isolated bacteria must be conducted through appropriate testing. If isolated colonies are still present, retesting is necessary.
  • Invert the agar plate, hold it against the light, and observe the bottom surface of the plate. Use a caliper to measure the complete diameters of the inhibition zones (including the antibiotic disks). Read the zone edges from the area where bacterial growth is not observed with the naked eye. Any faint or small colonies at the edge of the growth inhibition zone can be overlooked when magnified.
  • For interpretation of inhibition zones, refer to CLSI M100 guidelines for each bacterial strain.

The relevant samples are directly inoculated onto the agar plates.

  • If the agar plates have been refrigerated, allow them to warm up to room temperature before inoculating the samples.
  • Inoculate the samples onto the agar plates.
  • Incubate the plates in an aerobic condition at 35 – 37°C for 16 – 24 hours.

BacterLab conducts quality control for each batch of products using standard ATCC strains under specific incubation conditions. The expected results for different ATCC strains are as follows:

Standard Strain incubation conditions Cultivation result
Enterococcus faecalis ATCC 33186 24 hours at 35°C Good growth, with zone sizes conforming to CSLI guidelines.
Escherichia coli ATCC 25922 16-18 hours at 35°C Good growth, with zone sizes conforming to CSLI guidelines.
Staphylococcus aureus ATCC 25923  24 hours at 35°C Good growth, with zone sizes conforming to CSLI guidelines.
Pseudomonas aeruginosa ATCC 27853 16-18 hours at 35°C Good growth, with zone sizes conforming to CSLI guidelines.

The components per 1 liter (reference):

Acid Digest of Casein 17.5g
Beef Extract 2g
Starch 1.5g
Sodium chloride 20g
Agar 15g
The pH of the complete medium at 25°C is 7.3 ± 0.1.

*Note: This composition is for reference purposes only. In addition, our company will prepare the components according to specific requirements or tender documents.

Product name Product code Packing
TraceMedia™ Mueller Hinton Agar + NaCl TM3222 10 Dishes/box, 20 Dishes/box

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