TraceMedia™ TCBS/ SS

  • Product Code: TM11222.
  • Packaging: Available in two options, with 10 dishes per box and 20 dishes per box.
  • Storage: Keep refrigerated at a temperature of 2 – 8°C.
  • Transportation: Transport at room temperature.
  • Expiration date: The product is valid for 03 months from the date of manufacture.
  • TraceMedia™ TCBS/ SS is a pre-poured agar medium on 90mm petri dishes used for culturing and isolating Vibrio, Salmonella, and Shigella from clinical specimens and research purposes.
PURPOSE OF USE:
  • TraceMedia™ TCBS/ SS consists of Thiosulfate Citrate Bile Salts Sucrose Agar (TCBS) and Salmonella Shigella Agar (SS).
  • Thiosulfate Citrate Bile Salts Sucrose Agar (TCBS): This medium is used for selective culturing to isolate Vibrio. It differentiates V. cholerae from other Vibrio species based on their ability to ferment sucrose.
  • Salmonella Shigella Agar (SS): This selective medium is used for the isolation and differentiation of Salmonella and Shigella.
PRINCIPLES:
  • TraceMedia™ TCBS/ SS contains proteose peptone and yeast extract, providing nitrogen compounds, complex B vitamins, and other essential growth nutrients while inhibiting gram-positive bacteria and coliforms. Sodium thiosulfate acts as a good sulfur source, combined with iron citrate, which detects the production of hydrogen sulfide. Bromothymol blue and thymol blue serve as pH indicators.
  • The alkaline pH of the medium enhances the recovery of V. cholerae and V. alginolyticus, resulting in yellow colonies on TCBS Agar due to sucrose fermentation. V. parahaemolyticus and V. vulnificus do not ferment sucrose and produce blue-green colonies.
  • SS Agar is a selective medium in which gram-positive bacteria are inhibited by bile salts, resulting in vibrant green and sodium citrate. Peptone provides a source of nitrogen and carbon, long-chain amino acids, vitamins, and necessary nutrients for growth. Lactose is a fermentable carbohydrate. The vibrant green color, bile salts, and thiosulfate selectively inhibit gram-positive bacteria and coliforms. Some enteric organisms reduce sodium thiosulfate to form sulfite and H2S gas.
INTERPRETATION OF RESULTS:
  • After incubation for the specified time, typically 16-24 hours, observe the colony growth on the surface of the petri dish.
  • Further identification of isolated bacteria requires appropriate testing methods.

The relevant samples are directly inoculated onto the agar plates.

  1. If the agar plates have been refrigerated, allow them to warm up to room temperature before inoculating the samples.
  2. Inoculate the samples onto the agar plates.
  3. Incubate the plates in an aerobic condition at 35 – 37°C for 16 – 24 hours.

BacterLab performs quality testing on each batch of its products using standard ATCC strains. The results of the culture are as follows:

Microorganism strain Strain incubation conditions Growth result
TCBS Medium:
Vibrio cholerae ATCC 15748 35-370C, 16-24h  Good growth, forming yellow colonies
Escherichia coli ATCC 25922 Weak growth to no growth
SS Medium:
Shigella flexneri ATCC 12022 35-370C, 16-24h Good growth, forming colorless colonies
Escherichia coli ATCC 25922 Weak growth to no growth

* The incubation temperature and time for both media are 35-37°C for 16-24 hours. The strains of Escherichia coli ATCC 25922 are used as controls in both TCBS and SS media.

The reference composition for 1 liter of TCBS medium is as follows:

TCBS medium
Proteose peptone 10,0 g
Yeast extract 5,0 g
Sodium thiosulphate 10,0 g
Sodium citrate 10,0 g
Bile 8,0 g
Sucrose 20,0 g
Sodium chloride 10,0 g
Ferric citrate 1,0 g
Bromo thymol blue 0,04 g
Thymol blue 0,04 g
pH of the complete medium at 25°C: 8.6 ± 0.2
SS Medium:
Peptone 5,0 g
Lactose 10,0 g
Bile salts mixture 8,5 g
Sodium citrate 10,0 g
Sodium thiosulphate 8,5 g
Ferric citrate 1,0 g
Brilliant green 0,00033 g
Neutral red 0,025 g
pH of the complete medium at 25°C: 7.0 ± 0.2

* Please note that these compositions are for reference only. Our company can customize the components according to specific requirements or tender documents.

Product name Product code Packing
TraceMedia™ TCBS/ SS TM11222 10 Petri dishes/box; 20 Petri dishes/box

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