{"id":1974,"date":"2023-08-11T08:31:51","date_gmt":"2023-08-11T08:31:51","guid":{"rendered":"https:\/\/labone.vn\/bacterlab\/?post_type=product&#038;p=1974"},"modified":"2025-04-04T02:59:16","modified_gmt":"2025-04-04T02:59:16","slug":"bacterplate-columbia-agar-cba-plates","status":"publish","type":"product","link":"https:\/\/labone.vn\/bacterlab\/en\/product\/bacterplate-columbia-agar-cba-plates\/","title":{"rendered":"BacterPlate\u2122 Columbia Agar (CBA)"},"content":{"rendered":"<div><span style=\"font-size: 100%;\"><strong><span style=\"color: #3366ff;\">INTENDED USE<\/span><\/strong><\/span><\/div>\n<ul>\n<li><strong>BacterPlate\u2122 Columbia Agar<\/strong> is a highly nutritive medium used for the growth and isolation of a large variety of microorganisms, particularly very fastidious bacteria: streptococci and pneumococci in animal samples. When blood, selective agents or growth accelerators are added, it becomes possible to prepare a wide variety of media adapted to specific uses.<\/li>\n<li>The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.<\/li>\n<\/ul>\n<p><span style=\"font-size: 100%;\"><strong><span style=\"color: #3366ff;\">PRINCIPLES<\/span><\/strong><\/span><\/p>\n<ul>\n<li>Peptones included in the composition of the medium favor the excellent growth of colonies.<\/li>\n<li>Yeast extract is a source of vitamin B complex.<\/li>\n<li>Starch is a detoxifying agent and also an energy source.<\/li>\n<li>Defibrinated sheep blood, which can be added to the medium, favors the detection of hemolytic reactions and supplies X factor (heme) required for the growth of a large number of bacteria, but lacks V factor (nicotinamide adenine dinucleotide) due to the presence of an NADase, which destroys any NAD present. Haemophilus influenzae, which requires both X and V factors, does not grow on agar containing ordinary blood.<\/li>\n<li>The following media can be prepared with Columbia base :\n<ul>\n<li>Blood agar: By adding 5 or 10% sterile sheep blood after autoclaving and cooling, the medium is suitable for the growth of <em>Streptococcus<\/em>, <em>Pneumococcus<\/em>, <em>Staphylococcus<\/em>, <em>Listeria<\/em> and <em>Erysipelothrix<\/em>. It can be made selective by adding colistin and nalidixic acid to preclude the development of Gram-negative bacteria and <em>Bacillus<\/em>.<\/li>\n<li>Chocolate agar: By adding 10% sheep or horse blood to sterile Colombia Agar and heating to 70\u00b0C for 5 minutes until a chocolate color develops, an excellent medium is obtained for growth of <em>Haemophilus<\/em>, <em>Neisseria<\/em>, <em>Taylorella<\/em> or <em>Campylobacter<\/em>.<\/li>\n<li>Base media without enrichment: Columbia Agar can be used to grow <em>Brucella abortus<\/em>, <em>Yersinia pestis<\/em> and <em>Clostridium perfringens<\/em>, as well as all enterobacteria<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<ul>\n<li>Ready-to-use chromogenic medium on 90mm plates for culturing and isolating of fastidious microorganisms<\/li>\n<li><strong>Code:<\/strong> <strong>05008<\/strong><\/li>\n<li><strong>Packaging:<\/strong> 10 plates\/ box or as per customer request.<\/li>\n<li><strong>Storage Conditions:<\/strong> 2\u20138\u00b0C.<\/li>\n<li><strong>Transportation:<\/strong> Ambient temperature.<\/li>\n<li><strong>Shelf Life:<\/strong> 03 months from the date of manufacture.<\/li>\n<\/ul>\n","protected":false},"featured_media":2174,"template":"","meta":[],"product_cat":[335],"product_tag":[],"class_list":["post-1974","product","type-product","status-publish","has-post-thumbnail","hentry","product_cat-microbiological-culture-medium"],"_links":{"self":[{"href":"https:\/\/labone.vn\/bacterlab\/wp-json\/wp\/v2\/product\/1974","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/labone.vn\/bacterlab\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/labone.vn\/bacterlab\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/labone.vn\/bacterlab\/wp-json\/wp\/v2\/media\/2174"}],"wp:attachment":[{"href":"https:\/\/labone.vn\/bacterlab\/wp-json\/wp\/v2\/media?parent=1974"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/labone.vn\/bacterlab\/wp-json\/wp\/v2\/product_cat?post=1974"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/labone.vn\/bacterlab\/wp-json\/wp\/v2\/product_tag?post=1974"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}