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BacterChrom™ Staphylococcus Agar Plates
- Ready-to-use chromogenic medium on 90mm plates for isolating Staphylococcus spp. in industrial products, veterinary samples, and environmental samples.
- Code: 01019
- Packaging: 10 plates/box or as per customer requirements.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
INTENDED USE
- BacterChrom™ Staphylococcus Agar is a selective chromogenic medium designed for the isolation and direct differentiation of Staphylococcus species from industrial products, veterinary samples, and environmental specimens. This medium enables the identification of target microorganisms based on colony color and morphology, providing results after 18 – 24 hours of incubation at 37°C under aerobic conditions.
- It is intended for research and diagnostic purposes in industrial, veterinary, and environmental testing settings..
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- BacterChrom™ Staphylococcus Agar facilitates the differentiation of Staphylococcus species through chromogenic reactions based on specific enzymatic activities. These reactions produce distinct colony colors, enabling easy presumptive identification. The medium also includes selective agents to inhibit the growth of non-target organisms, ensuring specificity and minimizing interference.
- Allow to warm to room temperature before inoculation, dry the plates in an incubator with the covers partially removed.
- Direct streaking sample onto the plate
- Incubate at 37°C in aerobic conditions and read at 18h and 24h.
RESULTS
- Qualitative reading and interpretation of the plates:
| Microorganism | Typical colony apprearance |
| S. aureus | pink to mauve |
| S. epidermidis | colourless to pinkish |
| S. saprophyticus | turquoise blue |
| Other Staphylococci | various |
| Streptococci | inhibited |
| Gram (-) bacteria | inhibited |
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| S. aureus ATCC 25923 | Incubate for 18 – 24 hours at 35 – 37 °C | Mauve colonies |
| S. saprophyticus ATCC 15305 | Turquoise blue colonies | |
| E. coli ATCC 35218 | Inhibited | |
| C. albicans ATCC 10231 | Inhibited | |
| E. faecalis ATCC 29212 | Inhibited |
For 1 liter of medium
| Peptones | 19,4 g |
| Activators | 21,7 g |
| Buffering system | 1,4 g |
| Selective system | 4,5 g |
| Bacteriological agar | 14,4 g |
| Bovine fibrinogen | 5,3 g |
| Rabbit plasma, EDTA | 25 mL |
| Trypsin inhibitor | 25 mg |
| Potassium tellurite | 25 mg |
pH of the ready-to-use medium at 25°C: 7,3 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterChrom™ Staphylococcus Agar | 01019 | 10 plates/box or as per customer request. |
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