BacterChrom™ Yeast, Mold Agar

Ready to use chromogenic medium on 90mm plates or quantifying yeasts and molds in food and water.
Code: 01001
Packaging: 10 plates/box or as per customer requirements.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 03 months from the date of manufacture.

INTENDED USE

BacterChrom™ Yeast, Mold Agar allows the enumeration of yeasts and molds in all human and animal food products regardless of their water activity. It can also be used for the control of environmental samples in production areas. In the case of water samples, they can be analyzed by membrane filtration using this media.

PRINCIPLES

The choice of peptones, carbohydrates and growth promotors were specially selected in order to optimize the rapid growth of yeasts & molds.
Rose Bengal is assimilated by yeasts which facilitate their enumeration by coloring them pink.
The selective system, associated with the pH of the media, insures the inhibition of the majority of bacterial contaminants.
The media has been conceived in a way that reduces the propagation of Mucor thallus, which facilitates their count after only 54 hours of incubation. It is also well adapted to the enumeration of mold spores

Allow to warm to room temperature before inoculation, dry the plates in an incubator with the covers partially removed.
Transfer 0,1 mL of the sample to be tested and its serial dilutions to the surface of the ready-to-use plates.
To estimate low numbers, inoculate 1 mL of initial suspension on to the surface of 3 plates
Spread the inoculum over the surface of the plate with a sterile triangle or “hockey stick”.
Incubate the plates with the cover facing up at 25 °C for 54 to 72 hours.

RESULTS

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

Reference strains	Incubation conditions	Expected results
Saccharomyces cerevisiae ATCC 20827	Incubate for 72h at 25 ± 1^oC	PR ≥ 50 %
Candida albicans ATCC 10231		PR ≥ 50 %
Aspergillus brasiliensis ATCC 16404		PR ≥ 50 %
Escherichia coli ATCC 25922		Inhibited
Bacillus subtilis ssp. Spizizenii ATCC 6633		Inhibited

For 1 liter of medium

pH of the ready-to-use medium at 25°C: 5,6 ± 0,2

*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.

Product name	Code	Packaging
BacterChrom™ Yeast, Mold Agar	01001	10 plates/ box or as per customer request.