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StickSpongesCollect™ Polyurethane Buffered Peptone Water with neutralizers, 10 mL
- Microbial Sampling Tool for surface sampling, using a plastic stick with an attached polyurethane sponge pre-moistened with Buffered Peptone Water containing neutralizers, used for monitoring surfaces and verifying sterilization and disinfection effectiveness.
- Code: 407010102710
- Packaging: 100 pieces/box (5 bags) or customer request.
- Storage Conditions: 4 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 12 months from the date of manufacture.
INTENDED USE
- StickSpongesCollect™ Polyurethane Buffered Peptone Water with neutralizers, 10 mL is intended for microbiological monitoring of surfaces and environmental objects, as well as for confirming and evaluating the effectiveness of sterilization and disinfection processes. The product facilitates the collection of bacterial samples from surfaces, especially from hard-to-reach areas such as drains, equipment, and surrounding areas in food manufacturing facilities. It is suitable for use in medical, pharmaceutical, cosmetic, food, and other hygiene-sensitive industries
PRINCIPLES
- StickSpongesCollect™ Polyurethane Buffered Peptone Water with neutralizers, 10 mL utilizes a plastic stick with an attached biocide-free polyurethane sponge combined with 10 mL of Buffered Peptone Water containing neutralizer to collect microorganisms from surfaces. The Buffered Peptone Water with neutralizing agents effectively neutralizes residual disinfectants and sanitizers on the sampled surfaces, supporting the recovery and preservation of microorganisms during sample collection, transport, and analysis. The sponge material and moisture content ensure optimal microbial viability and stability.
- Inspect the StickSpongesCollect™ product to ensure there is no damage or contamination during storage.
- Write sample information on the provided label attached to the product packaging.
- Wear the provided sterile gloves (if available).
- Tear the bag along the pre-marked perforated line.
- Remove the StickSpongesCollect™ stick by pulling the bag from the outside. Be careful not to touch the inside of the bag.
- Rub the StickSpongesCollect™ sponge on the area to be sampled.
- After sampling, return the sampling stick to the bag, making sure not to insert it too deep to avoid touching the inside of the bag.
- Remove the sponge from the stick by holding the sponge from the outside of the bag and gently shaking until the sponge comes off the stick.
- Use the provided tie to seal the StickSpongesCollect™ bag by rolling the top of the bag and tightly twisting the tie inside the bag..
- The sample must be sent to the laboratory as soon as possible to prevent changes in microbial characteristics. The transportation process should comply with sample storage regulations.
- StickSpongesCollect™ Polyurethane Buffered Peptone Water with neutralizers, 10 mL is produced in a closed environment, adhering to strict hygiene regulations. The polyurethane sponge is biocide-free and sterilized using gamma irradiation, ensuring no contaminants affect the sample quality.
- BacterLab ensures the quality of each product batch of the medium by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| S. Typhimurium ATCC 14028 | 35 – 37°C for 18 – 24 hours | Good growth |
| E. coli ATCC 35218 | Good growth | |
| S. aureus ATCC 25923 | Good growth |
1. Stick sponges
- Material: Biocide-free polyurethane
- Size: 1.5″ x 3″
- Stick handle: PP plastic
2. Medium
- Volume of solution impregnated into sponges: 10 mL
- Components of the medium:
For 1 liter of medium
| Bacteriological peptone | 10,0 g |
| Sodium Choloride | 5,0 g |
| Sodium phosphate dibasic 12-hydrate | 9,0 g |
| Potassium phosphate monobasic anhydrous | 1,5 g |
| Tween 80 (Polysorbate 80) | 3 mL |
| Histidine | 0,1 g |
| Sodium thiosulfate | 0,8 g |
| Soy lecithin | 0,3 g |
pH of the ready-to-use medium at 25°C: 7,0 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| StickSpongesCollect™ Polyurethane Buffered Peptone Water with neutralizers, 10 mL | 407010102710 | 100 pieces/box (5 bags) or customer request. |
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