TraceMedia™ Cetrimide Agar

  • Product Name: TraceMedia™ Pseudomonas aeruginosa Isolation and Differentiation Agar.
  • Product Code: TM3022.
  • Packaging: 10 Discs/Box.
  • Storage: 2 – 8°C.
  • Transportation: Ambient temperature.
  • Expiration Date: 03 months from the date of production.
INTENDED USE
  • TraceMedia™ Cetrimide Agar is used for the selective cultivation and differentiation of Pseudomonas aeruginosa. Cetrimide is a quaternary ammonium compound with antibacterial activity against a range of Gram-positive organisms and some Gram-negative organisms.
  • Pseudomonas aeruginosa produces several water-soluble iron-chelating agents, including yellow-green or brownish fluorescent pyoverdin. When pyoverdin combines with water-soluble blue-green pyocyanin, the characteristic bright bluish-green color of Pseudomonas aeruginosa is produced. The addition of magnesium chloride and potassium sulfate enhances the production of these iron-chelating agents.

RESULTS:

  • After incubation, observe the growth of bacteria and the presence of colonies:
  • Pseudomonas aeruginosa colonies appear yellow-green or brownish and fluoresce under UV light. Presumptive identification based on colony morphology needs to be confirmed using further tests such as the oxidase test and subculture on media to detect pyoverdin and pyocyanin.

MEDIA CHARACTERISTICS:

  • Homogeneity: The medium is uniform in color, non-clumping, and does not separate into layers.
  • Medium color: Pale greenish-blue agar, slightly opaque.
  • Sterility: No bacterial/fungal growth after incubation at 36 ± 1°C for 48 – 72 hours.
STORAGE AND TRANSPORTATION CONDITIONS:
  • Storage: Store at 2 – 8°C.
  • Transportation: Ambient temperature.
PACKAGING SPECIFICATIONS:
  • Packaging: 10 discs/box.

EXPIRATION DATE:

  • Expiration Date: 03 months from the date of production.
1. To ensure the stability of the culture media discs at room temperature, dry the discs in an incubator by partially opening the lid of the petri dishes.
2. Transfer 0.1 mL of the sample to be tested, with different dilutions already prepared, onto the surface of the agar medium. Spread the liquid evenly on the surface of the agar using a triangular inoculation loop.
3. Alternatively, you can streak the sample on the agar medium using a loop or a loopful of the sample can be streaked in a circular pattern on the plates.
4. Incubate the petri dishes at 36 ± 1°C for 18 – 24 hours.

BacterLab tests the quality of each batch of products using ATCC standard strains:

Microorganism Strain Growth after 16-24 hours Growth at 35-37°C
Pseudomonas aeruginosa ATCC14207 Good growth, produces fluorescent yellow-green to blue-green colonies
Escherichia coli ATCC 8739 No growth or weak growth

Ingredients per 1 liter (Reference):

Gelatin peptone 20 g
Magnesium Chloride 1.4 g
Potassium Sulphate 10 g
Cetrimide 0.3 g
Agar 13.6 g
pH of the complete medium at 25°C: 7.3 ± 0.2

*Please note that this composition is for reference purposes. Additionally, Our company will prepare the components as required or according to the tender documents.

Product name Product code Packing
TraceMedia™ Cetrimide Agar TM3022 10 Discs/Box

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