- TraceMedia™ MacConkey Agar is a medium used for selective cultivation and differentiation of lactose-fermenting Gram-negative bacteria that are easy to grow. It is applied to isolate infectious agents from various clinical specimens.
- TraceMedia™ MacConkey Agar can differentiate between bacteria that ferment lactose and those that do not.
- Lactose-fermenting bacteria produce acid, which lowers the pH of the medium, causing it to turn pink or red. Additionally, the acid formed from lactose fermentation reacts with bile salts, forming precipitates, resulting in a bright pink halo around the lactose-fermenting colonies.
- Lactose-weak fermenters may still turn the medium pink or red, but they do not produce a halo around the colonies.
- Non-lactose-fermenting bacteria will keep the original color of the medium, and the colonies will not be colored.
- After incubation for the required time, typically 16-24 hours, observe bacterial growth on the surface of the plate.
- Further identification of isolated bacteria should be performed using appropriate tests.