TraceMedia™ MacConkey Agar

  • Shigella and Salmonella Isolation Agar from Gastrointestinal Specimens.
  • Product Code: TM1622.
  • Pack Size: 10 Discs/Box, 20 Discs/Box.
  • Storage: 2 – 8°C.
  • Transportation: Ambient temperature.
  • Expiration Date: 03 months from the date of manufacture.
INTENDED USE
  • TraceMedia™ MacConkey Agar is a medium used for selective cultivation and differentiation of lactose-fermenting Gram-negative bacteria that are easy to grow. It is applied to isolate infectious agents from various clinical specimens.

PRINCIPLE

  • TraceMedia™ MacConkey Agar can differentiate between bacteria that ferment lactose and those that do not.
  • Lactose-fermenting bacteria produce acid, which lowers the pH of the medium, causing it to turn pink or red. Additionally, the acid formed from lactose fermentation reacts with bile salts, forming precipitates, resulting in a bright pink halo around the lactose-fermenting colonies.
  • Lactose-weak fermenters may still turn the medium pink or red, but they do not produce a halo around the colonies.
  • Non-lactose-fermenting bacteria will keep the original color of the medium, and the colonies will not be colored.
RESULTS
  • After incubation for the required time, typically 16-24 hours, observe bacterial growth on the surface of the plate.
  • Further identification of isolated bacteria should be performed using appropriate tests.
The relevant samples are directly inoculated onto the agar plate:
  • If the agar plate has been refrigerated, allow it to warm up to room temperature before inoculating the sample.
  • Inoculate the sample onto the agar plate.
  • Incubate the plate under aerobic conditions at 35 – 37°C for 16 – 24 hours.

BacterLab performs quality control on each batch of products using ATCC standard strains:

Standard  Incubation at 35 – 37oC for 16 – 24 hours Result
Shigella flexneri ATCC 12022 Good growth, colonies appear beige in color
Escherichia coli ATCC 25922 Good growth, colonies appear pink in color
Staphylococcus aureus ATCC 25923 Weak to no growth observed

Ingredients per 1 liter (Reference):

Pancreatic Digest of Gelatin 17,0 g
Peptones (meat and casein) 3,0 g
Lactose 10,0 g
Bile Salts No.3 1,5 g
Sodium Chloride 5,0 g
Agar 13.5 g
Neutral Red 0,03 g
Crystal Violet 1,0 mg
pH of the complete medium at 25°C: 7.1 ± 0.2

*Please note that this composition is for reference purposes. Additionally, Our company will prepare the components as required or according to the tender documents.

Product name Product code Packaging
TraceMedia™ MacConkey Agar TM1622 10 plates/box; 20 plates/box

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