Vn
Dilution and enrichment buffer
Antibiotic disc dispenser
INTENDED USE
- TraceMedia™ Mannitol Salt Agar is a pre-poured agar medium in 90mm petri dishes used for selective cultivation of Staphylococcus and differentiation of Staphylococcus aureus and Staphylococcus epidermidis.
PRINCIPLES
- TraceMedia™ Mannitol Salt Agar is a selective medium prepared for the isolation of pathogenic cocci.
- Most other bacteria are inhibited by the high salt concentration, except for some halophilic bacteria.
- Coagulase-positive cocci produce colonies surrounded by bright yellow zones, while coagulase-negative cocci produce colonies with purple-red zones.
RESULTS
- After incubation for the required time, usually 18-24 hours, observe the growth of colonies on the agar surface with characteristic features such as colony size, color, and morphology.
- Further identification of the isolated bacteria must be conducted through appropriate tests.
The relevant samples are directly inoculated onto the agar plates.
- If the agar plates have been refrigerated, allow them to warm up to room temperature before inoculating the samples.
- Inoculate the samples onto the agar plates.
- Incubate the plates in an aerobic condition at 35 – 37°C for 16 – 24 hours.
BacterLab tests the quality of each batch of products using standard strains from ATCC:
| Test Microorganisms | Incubation Conditions | Quality Requirement |
| Staphylococcus aureus ATCC® 25923 * | 35 – 370C 16 – 24 giờ |
Good growth, colonies appear yellow |
| Staphylococcus epidermidis ATCC® 12228 * |
Good growth, colonies appear pink | |
| Escherichia coli ATCC® 8739 * | No growth |
Ingredients per 1 liter (Reference):
| `Lab-Lemco’ powder | 1.0 |
| Peptone | 10.0g |
| Mannitol | 10.0g |
| Sodium chloride | 75.0g |
| Phenol red | 0.025g |
| Agar | 15.0g |
| pH 7.5 ± 0.2 @ 25°C | |
*Please note that this composition is for reference purposes. Additionally, Our company will prepare the components as required or according to the tender documents.
| Product name | Product code | Paking |
| TraceMedia™ Mannitol Salt Agar | TM1922 | 10 plates/box; 20 plates/box |
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