Vn
Dilution and enrichment buffer
Antibiotic disc dispenser
- The pre-poured agar media in 90mm Petri dishes is used for the screening and detection of ESBL (Extended-spectrum β-lactamase) and MRSA (Methicillin Resistant Staphylococcus Aureus) bacteria.
INTENDED USE:
- TraceMedia™ MRSA/ VRE is a chromogenic media used for the detection and differentiation of MRSA and ESBL-producing bacteria.
PRINCIPLE:
TraceMedia™ MRSA/ VRE:
- The MRSA section allows for direct detection and identification of MRSA through a combination of chromogenic substrates and cefoxitin.
- MRSA bacteria will grow and produce pink to pale purple colonies due to the hydrolysis of the chromogenic substrate.
- Selective agents prevent the growth of Gram-negative bacteria, fungi, and some other Gram-positive cocci.
- The ESBL section is a chromogenic media used to detect Gram-negative bacteria that produce beta-lactamase enzymes resistant to penicillins, third-generation cephalosporins (C3G), and monobactams.
- The ESBL media contains a mixture of peptone as a source of nutrients for bacterial growth. Chromogenic and selective mixes aid in the selection and visualization of ESBL-producing bacteria.
READING RESULTS:
- After incubation for the specified time, typically 16-24 hours, observe the bacterial growth on the surface of the plate.
- Further identification of isolated bacteria should be performed using appropriate tests.
The relevant samples are directly inoculated onto the agar plates.
- If the agar plates have been refrigerated, allow them to warm up to room temperature before inoculating the samples.
- Inoculate the samples onto the agar plates.
- Incubate the plates in an aerobic condition at 35 – 37°C for 16 – 24 hours.
BacterLab performs quality control on each batch of its products using standard ATCC strains:
| Microorganism strain | Strain incubation conditions | Growth result |
| MRSA compartment | ||
| Staphylococcus.aureus ATCC 43300 | 35 – 370C 16-24h |
Good growth, colonies appear as pale pink. |
| Staphylococcus aureus ATCC 25923 | Weak growth or no growth observed. | |
| Escherichia coli ATCC 25922 | Weak growth or no growth observed. | |
| ESBL compartment | ||
| Klebsiella.pneumoniae ATCC 700603 | 35 – 370C, 16-24h |
Good growth, blue bacteria |
| Escherichia coli ATCC 25922 | Weak growth or no growth observed. | |
The reference compositions for 1 liter:
| MRSA Medium: | |
| Chromopeptone | 35,0 g |
| Sodium chloride | 17,5 g |
| Inhibitory Agents | 7,52 g |
| Chromogen Mix | 0,5 g |
| Cefoxitin | 5,2 mg |
| Agar | 14,0 g |
| pH of the complete medium at 25°C: 7.2 ± 0.2 | |
| ESBL Medium: | |
| Agar | 15,0 g |
| Peptone and yeast extract | 17,0 g |
| Chromogenic mix | 1,0 g |
| Selective mix | 0,57 g |
| pH of the complete medium at 25°C: 7.0 ± 0.2 | |
*Please note that these compositions are for reference only. Our company can customize the components according to specific requirements or tender documents.
| Product name | Product code | Packing |
| TraceMedia™ MRSA/ VRE | TM11422 | 10 plates/box |
Related products
TraceMedia™ Columbia Sheep/Macconkey (BA/MC 90mm)
- Apply isolation of infectious agents from various types of clinical specimens.
- Code: TM10822.
- Packaging: 10 plates/ box.
- Storage: 2 – 8°C.
- Transportation: Room temperature.
- Shelf life: 03 months from the date of manufacture.
TraceMedia™ Columbia Sheep/Chocolate Bacitracin
- Product code: TM10922.
- Packaging options: 10 plates/box; 20 plates/box.
- Recommended storage condition: 2 – 8°C.
- Transportation temperature: Room temperature.
- Shelf life: 03 months from the date of manufacture.
TraceMedia™ MacConkey Agar/ Sabouraud Agar
- Product Code: TM11022.
- Package Size: 10 discs/box.
- Storage: 2 – 8°C.
- Transport: Room temperature.
- Expiration Date: 03 months from the date of manufacture.
TraceMedia™ MacConkey Agar/ Samonella Shigella Agar
- Applying isolation of infectious agents from fecal specimens.
- Code: TM11122.
- Packaging: 10 plates/box.
- Storage: 2 – 8°C.
- Transport: Ambient temperature.
- Expiration date: 03 months from the date of manufacture.
TraceMedia™ TCBS/ SS
- Product Code: TM11222.
- Packaging: Available in two options, with 10 dishes per box and 20 dishes per box.
- Storage: Keep refrigerated at a temperature of 2 – 8°C.
- Transportation: Transport at room temperature.
- Expiration date: The product is valid for 03 months from the date of manufacture.
TraceMedia™ ESBL/ MRSA
To apply for screening to detect ESBL (Extended Spectrum β-Lactamase) and MRSA (Methicillin-resistant S. aureus) bacteria, you can use the following product:
- Product name: TraceMedia™ ESBL/MRSA Screening Agar.
- Product code: TM11322.
- Packaging: 10 Petri dishes per box; 20 Petri dishes per box.
- Storage: Store at 2 – 8°C.
- Transport: Transport at room temperature.
- Expiration Date: 03 months from the date of manufacture.