Vn
Dilution and enrichment buffer
Antibiotic disc dispenser
- TraceMedia™ Mueller Hinton Blood Agar is a pre-poured petri dish, 90mm in diameter, used for testing antibiotic sensitivity or Minimum Inhibitory Concentration (MIC) using difficult-to-culture bacteria.
PURPOSE:
- TraceMedia™ Mueller Hinton Blood Agar is used to test antibiotic sensitivity or MIC using difficult-to-culture bacteria.
PRINCIPLE:
- TraceMedia™ Mueller Hinton Blood Agar contains a nutrient-rich composition that includes beef extract and acid hydrolysate of casein, providing amino acids, nitrogenous compounds, vitamins, and essential mineral salts for the growth of microorganisms. Starch is added to act as a protective colloid against toxic substances in the environment. Additionally, the medium contains a small amount of thymidine and thymine, and the concentration of divalent cations in the medium is carefully controlled when performing antibiotic susceptibility testing on the Mueller Hinton Agar medium. Agar is used as a solidifying agent for the medium. Sheep blood is supplemented to enhance the growth of difficult-to-culture bacterial species.
READING RESULTS:
- After incubation for the required time, observe bacterial growth on the surface of the petri dish and the circular inhibition zones around antibiotic disks.
- To identify the isolated bacteria, appropriate tests must be carried out. If separate colonies of bacteria are still present, retesting is necessary.
- Invert the petri dish, hold it up to the light, and observe the bottom of the dish. Use a ruler with millimeter markings to measure the complete diameter of the inhibition zones (including the antibiotic disks). The edge of the inhibition zone should be read from the area where bacterial growth is not visually detectable.
- Bacteria with faint or small inhibition zones at the edge of the growth inhibition only visible under magnification should be disregarded.
- For interpretation of the inhibition zones, refer to CLSI M100 for each microbial species.
The related discs are directly inoculated onto the Mueller Hinton Blood Agar plate.
- If the agar discs have been refrigerated, allow them to warm to room temperature before inoculating the samples.
- Inoculate the microbial samples onto the surface of the Mueller Hinton Blood Agar plate.
- Incubate the plate under the conditions of 16-24 hours, 35°C, and 5% CO2.
BacterLab performs quality control on each batch of products using the ATCC standard strains:
| Standard | Strain incubation conditions | Cultivation result |
| Streptococcus pneumonia | 16 – 24 hours, 35°C, 5% CO2 | Good growth, inhibition zone sizes according to CSLI standards |
| ATCC 46919 |
The components per 1 liter (reference):
| Acid Digest of Casein | 17.5g |
| Beef Extract | 2g |
| Starch | 1.5g |
| Sodium chloride | 20g |
| Sheep Blood | 70ml |
| Agar | 15g |
| pH of complete medium at 25°C: 7.3 ± 0.2 | |
*Note: This composition is for reference purposes only. In addition, our company will prepare the components according to specific requirements or tender documents.
| Product name | Product code | Packing |
| TraceMedia™ Mueller Hinton Blood Agar | TM3322 | 10 Dishes/box, 20 Dishes/box |
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