- TraceMedia™ Mueller Hinton Blood Agar is a pre-poured petri dish, 90mm in diameter, used for testing antibiotic sensitivity or Minimum Inhibitory Concentration (MIC) using difficult-to-culture bacteria.
- TraceMedia™ Mueller Hinton Blood Agar is used to test antibiotic sensitivity or MIC using difficult-to-culture bacteria.
- TraceMedia™ Mueller Hinton Blood Agar contains a nutrient-rich composition that includes beef extract and acid hydrolysate of casein, providing amino acids, nitrogenous compounds, vitamins, and essential mineral salts for the growth of microorganisms. Starch is added to act as a protective colloid against toxic substances in the environment. Additionally, the medium contains a small amount of thymidine and thymine, and the concentration of divalent cations in the medium is carefully controlled when performing antibiotic susceptibility testing on the Mueller Hinton Agar medium. Agar is used as a solidifying agent for the medium. Sheep blood is supplemented to enhance the growth of difficult-to-culture bacterial species.
- After incubation for the required time, observe bacterial growth on the surface of the petri dish and the circular inhibition zones around antibiotic disks.
- To identify the isolated bacteria, appropriate tests must be carried out. If separate colonies of bacteria are still present, retesting is necessary.
- Invert the petri dish, hold it up to the light, and observe the bottom of the dish. Use a ruler with millimeter markings to measure the complete diameter of the inhibition zones (including the antibiotic disks). The edge of the inhibition zone should be read from the area where bacterial growth is not visually detectable.
- Bacteria with faint or small inhibition zones at the edge of the growth inhibition only visible under magnification should be disregarded.
- For interpretation of the inhibition zones, refer to CLSI M100 for each microbial species.