Vn
Dilution and enrichment buffer
Antibiotic disc dispenser
INTENDED USE
- TraceMedia™ Potato Dextrose Agar is recommended in standards applied to cosmetics. It can be used as a selective medium after the addition of chloramphenicol or for application testing. The medium supports the growth of yeast and mold.
- Typical composition is described in NF EN ISO 18416.
PRINCIPLE
- Glucose and potato extract are favorable for the growth of yeast and mold.
- Low pH inhibits most other microorganisms.
- Potato extract eliminates the need for using potato infusion.
RESULTS
- Count the total yeast and mold colonies.
Preparation of Aspergillus spores (NF EN ISO 11930):
Inoculate spore suspension or spore suspension into a mold broth.
- Incubate at 20-25 °C for 7-11 days.
- Assess the antimicrobial protection ability of a cosmetic product (NF EN ISO 11930).
- Antimicrobial Effectiveness Test:
Transfer 1 mL of the test sample confirmed to contain Aspergillus brasiliensis and the control sample into separate sterile Petri dishes.
- Add 15 mL of the test medium into each Petri dish.
- Mix evenly by swirling and allow to solidify.
- Incubate at 20-25 °C for 3-5 days.
Dry medium: White, free-flowing, and uniform powder.
Complete medium: Sabouraud Dextrose Agar.
Typical culture response:
| Microorganism | Incubation Temperature and Time | Growth (Performance: PR) |
| Aspergillus brasiliensis WDCM 00053 | 72 hours at 20-25 °C | PR ≥70 % |
| Candida albicans WDCM 00054 | 48 hours at 30-35 °C | PR ≥70 % |
| Product name | Product code | packing |
| TraceMedia™ Potato Dextrose Agar | TM18522 | 10 Petri dishes per box. |
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