Vn
Dilution and enrichment buffer
Antibiotic disc dispenser
INTENDED USE
- Pre-poured agar medium on 90mm Petri dishes used for selective cultivation of Salmonella and Shigella from fecal samples.
PRINCIPLE
- TraceMedia™ Salmonella Shigella Agar is a selective medium in which Gram-positive bacteria are inhibited by bile salts.
- The high concentration of Citrate and Sodium thiosulfate limits the growth of Coliform bacteria and inhibits the growth of Proteus.
- Lactose fermentation produces acid, indicated by the formation of red colonies.
- Lactose-negative bacteria form colorless colonies.
- When thiosulfate and citrate are present in the medium, hydrogen sulfide-producing bacteria will form colonies with a black center.
RESULTS
- After incubation for the required time, typically 18-24 hours, observe the colonies’ growth on the plate with characteristic features of size, color, and morphology.
- Lactose non-fermenting species will appear as colorless colonies, while lactose fermenting species on the surface of the medium will appear as pink-red colonies.
- Most Salmonella and Shigella do not ferment lactose, resulting in colorless or pale colonies.
- Salmonella is often distinguished by the presence of black centers due to the reaction with H2S-indicator.
- Further identification of isolated bacteria must be conducted through appropriate tests.
The relevant samples are directly inoculated onto the agar plates.
- If the agar plates have been refrigerated, allow them to warm up to room temperature before inoculating the samples.
- Inoculate the samples onto the agar plates.
- Incubate the plates in an aerobic condition at 35 – 37°C for 16 – 24 hours.
BacterLab tests the quality of each batch of products using standard strains from ATCC/WDCM
| Standard | Strain Incubation Conditions | Results | ||
| Time | Temperature | Air Environment | ||
| Salmonella typhimurium ATCC® 14028 | 18h – 24h | 35oC | Aerobic | Good growth, colorless colonies with black centers |
| Shigella flexneri ATCC® 12022 | 18h – 24h | 35oC | Aerobic | Good growth, colorless colonies |
| Escherichia coli ATCC® 25922 | 18h – 24h | 35oC | Aerobic | Partial inhibition, pink colonies |
| Enterococcus faecalis ATCC® 29212 | 18h – 24h | 35oC | Aerobic | No growth |
Ingredients per 1 liter (Reference):
| ‘Lab-Lemco’ powder | 5,0 g |
| Peptone | 5,0 g |
| Lactose | 10,0 g |
| Bile salts | 8,5 g |
| Sodium citrate | 10,0 g |
| Sodium thiosulfate | 8,5 g |
| Ferric citrate | 1,0 g |
| Neutral red | 25,0 mg |
| Brilliant green | 0,33 mg |
| Agar | 15,0 g |
| pH of the complete medium at 25°C: 7.0 ± 0.2 | |
*Please note that this composition is for reference purposes. Additionally, Our company will prepare the components as required or according to the tender documents.
| Product name | Product code | packing |
| TraceMedia™ Salmonella Shigella Agar | TM1722 | 10 Petri dishes/box; 20 Petri dishes/box |
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