Vn
Dilution and enrichment buffer
Antibiotic disc dispenser
INTENDED USE
- TraceMedia™ TCBS Agar is a pre-poured petri dish medium of 90mm diameter used for the isolation and selection of Vibrio species. It allows the differentiation of Vibrio cholerae from other Vibrio species based on their ability to ferment sucrose.
PRINCIPLE
- TraceMedia™ TCBS Agar is designed for the isolation and selection of Vibrio cholerae and pathogenic Vibrio species associated with intestinal diseases. Thiosulfate, sodium citrate, and the alkaline pH of the medium significantly inhibit the growth of Enterobacteria.
- Beef extract and sodium cholate slow down the growth of enterococci and inhibit the growth of gram-positive bacteria.
- The medium’s acidic pH is the result of the sucrose fermentation by Vibrio, causing the bromothymol blue indicator to turn yellow.
- Bromothymol blue and thymol blue act as pH indicators. The presence of ferric citrate detects the production of hydrogen sulfide from thiosulfate as a sulfur source.
- Peptone and yeast extract provide nitrogen, vitamins, and amino acids in TCBS agar.
- Sodium chloride supports optimal growth and metabolic activity of Vibrio species living in a saline environment.
- Agar is used as a solidifying agent. The increased pH is used to enhance the growth of Vibrio cholerae, as this organism is sensitive to acidic conditions.
INTERPRETATION
- After incubation for the required time, usually 18-24 hours, observe the colonies developing on the surface of the agar plate.
- Further identification of the isolated bacteria must be conducted through appropriate tests.
The relevant samples are directly inoculated onto the agar plates.
- If the agar plates have been refrigerated, allow them to warm up to room temperature before inoculating the samples.
- Inoculate the samples onto the agar plates.
- Incubate the plates in an aerobic condition at 35 – 37°C for 16 – 24 hours.
BacterLab tests the quality of each batch of products using the ATCC standard strains:
| Standard | Strain Incubation Conditions | Results | ||
| Time | Temperature | Air Environment | ||
| Vibro cholerae ATCC 12022 | 18h – 24h | 35oC | Anaerobic | Well-developed growth, yellow colonies |
| Eschirichia coli ATCC 25922 | 18h – 24h | 35oC | Anaerobic | Weak growth to no growth |
Ingredients per 1 liter (Reference):
| Yeast extract powder | 5.0 g |
| Bacteriological peptone | 10.0 g |
| Sodium thiosulphate | 10.0 g |
| Tri-sodium citrate | 10.0 g |
| Ox bile | 8.0 g |
| Sucrose | 20.0 g |
| Sodium chloride | 10.0 g |
| Iron (III) citrate | 1.0 g |
| Bromothymol blue | 0.04 g |
| Thymol blue | 0.04 g |
| Agar | 14.0 g |
| pH of the complete medium at 25°C: 8.6 ± 0.2 | |
*Please note that this composition is for reference purposes. Additionally, Our company will prepare the components as required or according to the tender documents.
| Product name | Product code | Packing |
| TraceMedia™ TCBS Agar | TM2022 | 10 Petri dishes/box; 20 Petri dishes/box. |
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