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INTENDED USE
- BacterPlate™ XLD Agar is used for the isolation of Salmonella in pharmaceutical products. It can also be used as a second media of choice in the normalized methods for the detection of Salmonella in food products and water.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLE
- Sodium desoxycholate inhibits contaminating Gram-positive flora. Xylose is fermented by practically all enteropathogenic bacteria, except for Shigella which are thus differentiated from the other species. After exhausting xylose, Salmonella decarboxylate lysine (via lysine decarboxylase) to cadaverine, causing the pH to rise. Colonies of salmonellae resemble those of shigellae in the medium having become basic. The colonies formed are red in the presence of the indicator, phenol red.
- The addition of lactose and sucrose to the medium enable coliform bacteria to decarboxylate lysine and thereby produce excess acidity, making the indicator turn yellow, favoring their differentiation.
- In basic medium, pathogenic H2S-producers reduce ferric ammonium citrate and cause a blackening due to the production of iron sulfide at the center of the colonies. Non-pathogenic bacteria which do not decarboxylate lysine acidify the medium, a result of sugar fermentation. The pH decrease prevents the colonies from blackening.
- Re-inoculate a loop of enrichment media onto BacterPlate™ XLD Agar
- Incubate at 30 – 35 °C for 18 to 48 hours.
Note: For using XLD Agar as the second Salmonella isolation media, incubate the plates 24 hours at 37 °C.
RESULTS
- Salmonella present red colonies, with or without black centers.
- The aspect of the colonies is as follows:
| Microorganisms | Characteristics |
| Escherichia coli, Citrobacter, Enterobacter, Proteus, Serratia, Klebsiella | Yellow colonies, with or without a black center |
| Shigella, Providencia, Salmonella Paratyphi A | Red colonies without black center |
| Salmonella, Edwardsiella | Red colonies with black center |
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation Conditions | Expected results |
| E. faecalis ATCC 29212 | 35 ± 2oC for 18 – 24 hours | Inhibited, no growth |
| E.coli ATCC 35218 | Weak growth, yellow colonies | |
| S. Enteritidis ATCC 13076 | Good growth, red colonies with black centers |
For 1 liter of medium
| Yeast extract | 3,0 g |
| L-Lysine hydrochloride | 5,0 g |
| Lactose | 7,5 g |
| Sucrose | 7,5 g |
| Xylose | 3,75 g |
| Sodium desoxycholate | 1,0 g |
| Sodium chloride | 5,0 g |
| Sodium thiosulfate | 6,8 g |
| Ferric ammonium citrate | 0,8 g |
| Phenol red | 0,08 g |
| Agar | 12,5 g |
pH of the ready-to-use medium at 250C: 7,4 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterPlate™ XLD Agar | 05043 | 10 plates/ box or as per customer request. |
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