SpongesCollect™ Cellulose D/E Neutralizing Broth, 10 mL

Microbial Sampling Tool for surface sampling, using a cellulose sponge moistened with D/E Neutralizing Broth to neutralize the effects of residual disinfectants and protect microorganisms during transport and analysis
Code: 407010101110
Packaging:100 pieces/box (5 bags) or customer request.
Storage Conditions: 8 – 25°C.
Transportation: Ambient temperature.
Shelf Life: 6 months from the date of manufacture.

INTENDED USE

SpongesCollect™ Cellulose D/E Neutralizing Broth, 10mL is designed for microbiological sampling from surfaces in food processing, healthcare, and pharmaceutical environments. The primary purpose of this product is to support the collection of microbial samples from large, easily accessible surfaces and surrounding areas within food production facilities. D/E Neutralizing Broth helps neutralize the effects of disinfectants and protects microorganisms throughout transport and analysis.

PRINCIPLES

SpongesCollect™ Cellulose D/E Neutralizing Broth, 10mL uses a cellulose sponge combined with 10 mL of D/E Neutralizing Broth to collect microorganisms from surfaces. D/E Neutralizing Broth neutralizes the effects of disinfectants such as chlorine, quaternary ammonium compounds, iodine, phenolic compounds, mercury, formaldehyde, and glutaraldehyde, protecting microorganisms from their harmful effects. This medium provides the necessary nutrients to recover and promote microorganism growth, while maintaining their viability during transport. D/E Neutralizing Broth not only neutralizes disinfectants but also preserves the integrity of microorganisms, ensuring accurate analysis results. Bromocresol purple is included in the medium as a pH indicator, allowing monitoring of the neutralization process.

Inspect the SpongesCollect™ product to ensure there is no damage or contamination during storage.
Write sample information on the provided label attached to the product packaging.
Tear the bag along the pre-marked perforated line.
Push the sponge near the edge of the bag so that part of the sponge is exposed for easy handling. Hold the sponge from the outside of the bag and carefully remove it directly by hand, making sure not to touch the inside surface of the bag to avoid contamination.
Rub the SpongesCollect™ sponge on the area to be sampled.
After sampling, return the sponge to the bag. Ensure the sponge does not fall out and avoid direct contact with the outer surface of the packaging.
Use the provided tie to seal the SpongesCollect™ bag by rolling the top of the bag and tightly twisting the tie inside the bag.
The sample must be sent to the laboratory as soon as possible to prevent changes in microbial characteristics. The transportation process should comply with sample storage regulations.

SpongesCollect™ Cellulose Letheen Broth, 10mL is produced in a closed environment, adhering to strict hygiene regulations. The cellulose sponge is sterilized using gamma irradiation, ensuring no contaminants affect the sample quality.
LabCollect ensures the quality of each product batch of the medium by testing with ATCC reference strains.

1. Sponge

2. Medium

For 1 liter of medium

pH of the ready-to-use medium at 25°C: 7,6 ± 0,2

*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.

Product Name	Code	Packaging
SpongesCollect™ Cellulose D/E Neutralizing Broth, 10 mL	407010101110	100 pieces/box (5 bags) or customer request.