SpongesCollect™ Polyurethane Letheen Broth, 10 mL

Microbial Sampling Tool for surface sampling, using a polyurethane sponge moistened with Letheen Broth to neutralize the effects of disinfectants and protect microorganisms during transport and analysis
Code: 407010100410
Packaging:100 pieces/box (5 bags) or customer request.
Storage Conditions: 8 – 25°C.
Transportation: Ambient temperature.
Shelf Life: 6 months from the date of manufacture.

INTENDED USE

SpongesCollect™ Polyurethane Letheen Broth, 10mL is designed for microbiological sampling from surfaces in food processing, healthcare, and pharmaceutical environments. The primary purpose of this product is to support the collection of microbial samples from large, easily accessible surfaces and surrounding areas within food production facilities. Letheen Broth is a nutrient-rich pre-enrichment medium that helps test the effectiveness of disinfection processes while maintaining the viability and stability of microorganisms during transport and analysis.

PRINCIPLES

SpongesCollect™ Polyurethane Letheen Broth, 10mL uses a biocide-free polyurethane sponge combined with 10 mL of Letheen Broth to collect microorganisms from surfaces. Letheen Broth is a nutrient-rich medium containing lecithin and Tween 80. Lecithin neutralizes quaternary ammonium compounds, a key component in disinfectants, ensuring that microorganisms are not affected by residual disinfectants on surfaces. Tween 80 neutralizes disinfectants such as phenol, hexachlorophene, and formalin, protecting microorganisms from their harmful effects. This process helps recover and maintain the stability of microorganisms in food processing environments, ensuring they grow well throughout transport and analysis before being transferred to selective media without any alteration of their characteristics.

Inspect the SpongesCollect™ product to ensure there is no damage or contamination during storage.
Write sample information on the provided label attached to the product packaging.
Tear the bag along the pre-marked perforated line.
Push the sponge near the edge of the bag so that part of the sponge is exposed for easy handling. Hold the sponge from the outside of the bag and carefully remove it directly by hand, making sure not to touch the inside surface of the bag to avoid contamination.
Rub the SpongesCollect™ sponge on the area to be sampled.
After sampling, return the sponge to the bag. Ensure the sponge does not fall out and avoid direct contact with the outer surface of the packaging.
Use the provided tie to seal the SpongesCollect™ bag by rolling the top of the bag and tightly twisting the tie inside the bag.
The sample must be sent to the laboratory as soon as possible to prevent changes in microbial characteristics. The transportation process should comply with sample storage regulations.

SpongesCollect™ Polyurethane Letheen Broth, 10mL is produced in a closed environment, adhering to strict hygiene regulations. The biocide-free polyurethane sponge is sterilized using gamma irradiation, ensuring no contaminants affect the sample quality.
LabCollect ensures the quality of each product batch of the medium by testing with ATCC reference strains.

1. Sponge

2. Medium

For 1 liter of medium

pH of the ready-to-use medium at 25°C: 7,2 ± 0,2

*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.

Product Name	Code	Packaging
SpongesCollect™ Polyurethane Letheen Broth, 10 mL	407010100410	100 pieces/box (5 bags) or customer request.