Tiếng Việt
EXTENDED USE
- BacterChrom™ Salmonella Agar is a medium used to detect Salmonella in food, animal, and environmental samples. This medium has high specificity, capable of detecting atypical species and serovars such as S. Typhi, S. Paratyphi, S. Senftenberg, and lactose and saccharose fermenting strains. It also detects strains with weak or no esterase activity, such as S. bongori, S. Dublin, and some strains of S. enterica.
- This method has been NF VALIDATION certified according to the NF EN ISO 16140-2 (2016) standard for food products, animal feed, industrial environmental samples, and powdered milk.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
This method allows the detection of both motile and non-motile Salmonella.
- Enrichment Step: The sample is diluted 1:10 in Salmonella enrichment medium (Can be used: BacterBag™ Buffered Peptone Water), following the recommendations of the NF EN ISO 6579 standard. After adding the selective Supplement Salmonella to the enrichment medium, the solution turns green and is incubated for 16 – 24 hours at 41,5 ± 1,0°C.
- Differentiation Step: The enrichment culture is streaked onto BacterChrom™ Salmonella Agar plates and incubated for 16 – 24 hours at 37°C. Salmonella colonies appear magenta, while other bacteria typically form blue, purple, or colorless colonies.
- Confirmation Step: Classical tests described in standard methods can be performed or a direct latex test can be used from the magenta colonies isolated on the agar plate.
