BacterChrom™ LIN-R Plates

Ready-to-use chromogenic medium on 90mm plates for detecting and differentiating linezolid-resistant Gram (+) bacteria.
Code: 01026
Packaging: 10 plates/box or as per customer requirements.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 03 months from the date of manufacture.

INTENDED USE

BacterChrom™ LIN-R is a selective and differential chromogenic medium designed for the detection, isolation, and differentiation of Gram-positive bacteria resistant to linezolid (LIN-R). It is particularly intended for screening specimens such as nasal, rectal, and perianal swabs, as well as stool samples. The medium supports monitoring programs for antibiotic resistance, aiding in infection control efforts in healthcare environments
The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.

PRINCIPLES

BacterChrom™ LIN-R incorporates chromogenic substrates that react with specific enzymatic activities of linezolid-resistant Gram-positive bacteria, producing distinctive colony colors. Selective agents in the medium inhibit the growth of non-target organisms, ensuring specificity. The medium offers a rapid and efficient method for presumptive identification of resistant bacteria, simplifying laboratory workflows.

Allow to warm to room temperature before inoculation, dry the plates in an incubator with the covers partially removed.
Direct streaking sample onto the plate
Incubate at 35 – 37 °C for 36 – 48 hours in aerobic conditions.

RESULTS

Microorganism	Typical colony apprearance
LZD S.aureus	pink
LZD S.epidermidis	pink
LZD Enterococcus	steel blue
Other Gram-positive LZD bacteria	limited growth or inhibited
Gram-negative bacteria	inhibited

BacterLab ensures the quality of each product batch by testing with ATCC and NCTC reference strains.

Reference strains	Incubation conditions	Expected results
S. epidermidis LZD NCTC 13924	Incubate for 36 – 48 hours at 35 – 37 °C	Pink with pink halo colonies
E. faecalis LZD NCTC 14360		Steel blue with blue halo colonies
S. aureus ATCC 25923		Inhibited
E. faecalis ATCC 29212		Inhibited
E. coli ATCC35218		Inhibited

For 1 liter of medium

Peptone and yeast extract	20,0 g
Salt	7,0 g
Chromogenic mix and Selective mix	0,4 g
Growth factors	8 mL
Agar	15,0 g

pH of the ready-to-use medium at 25°C: 6,9 ± 0,2

*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.

Product Name	Code	Packaging
BacterChrom™ LIN-R	01026	10 plates/box or as per customer request.