BacterChrom™ Mycoplasma Plates

Ready-to-use chromogenic medium on 90mm plates for the detection of Mycoplasma bovis
Code: 01034
Packaging: 10 plates/box or as per customer requirements.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 03 months from the date of manufacture.

INTENDED USE

BacterChrom™ Mycoplasma is a selective and chromogenic culture medium designed for the detection and differentiation of Mycoplasma bovis in samples such as nasal swabs and broncho-alveolar lavage. The medium aids in the diagnosis of bovine infections caused by M. bovis, which may present as pneumonia, arthritis, mastitis, or keratoconjunctivitis.
This medium is primarily used in veterinary diagnostic laboratories and research environments.
The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.

PRINCIPLES

BacterChrom™ Mycoplasma utilizes chromogenic substrates that interact with specific enzymatic activities of M. bovis. Colonies of M. bovis are easily identifiable by their red coloration and characteristic “fried egg” morphology under a binocular loupe after 3–7 days of incubation at 37 °C in a CO₂ atmosphere. The medium also incorporates selective agents to inhibit the growth of non-target microorganisms, including Gram-positive and Gram-negative bacteria, yeasts, and molds. These features ensure high specificity, simplifying the identification process and supporting rapid and reliable diagnostic workflows.

Allow to warm to room temperature before inoculation, dry the plates in an incubator with the covers partially removed.
Direct streaking sample onto the plate
Incubate in aerobic conditions at 37°C for 3-7 days in a CO₂

RESULTS

Microorganism	Typical colony apprearance
Mycoplasma bovis	red with a fried egg morphology
Gram (+) bacteria	mostly inhibited
Gram (-) bacteria	inhibited
Yeast and mould	inhibited

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

Reference strains	Incubation conditions	Expected results
Mycoplasma bovis ATCC 25523	Incubate for 3 – 7 days at 35 – 37 °C, 5 – 10% CO₂	red with a fried egg morphology
Staphylococcus aureus ATCC 25923	Incubate for 3 – 7 days at 35 – 37 °C, 5 – 10% CO₂	inhibited

For 1 liter of medium

Peptone and yeast extract	25,0 g
Chromogenic mix and Selective mix	0,3 g
Mineral salt	6,3 g
Agar	10,0 g
Growth factor	2,2 g
Mineral salts	1,0 g
Chromogenic mix and Selective mix (supplement)	1,0 g
Growth factor (supplement)	150 mL

pH of the ready-to-use medium at 25°C: 7,6 ± 0,2

*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.

Product Name	Code	Packaging
BacterChrom™ Mycoplasma	01034	10 plates/box or as per customer request.