BacterPlate™ GNR/STAPH/STREP 3-Compartment Medium

    • Ready-to-use medium on 90mm plates for used to culture and test microorganisms.
    • Code: 05053
    • Packaging: 10 plates/ box or as per customer request.
    • Storage Conditions: 2–8°C.
    • Transportation: Ambient temperature.
    • Shelf Life: 03 months from the date of manufacture.

      INTENDED USE

      • BacterPlate™ GNR/STAPH/STREP 3-compartment medium is recommended for culturing and examining pathogenic microorganisms in medical, clinical, food, and cosmetic applications.
      • The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.

      PRINCIPLES

      • BacterPlate™ GNR/STAPH/STREP 3-compartment mediumconsists of three compartments with specific culture media:
        • GNR Compartment: Contains chromogenic Mastitis (GN) medium for isolating and differentiating Gram-negative pathogens such as:
          • pneumoniae: Blue-gray colonies
          • coli: Pink to dark red colonies
          • aeruginosa: Transparent colonies
          • Other microorganisms are inhibited.
        • STAPH Compartment: Contains chromogenic Staphylococcus medium for directly isolating and differentiating Staphylococcus:
          • aureus: Pink to lavender colonies
          • saprophyticus: Turquoise colonies
          • Other microorganisms are inhibited.
        • STREP Compartment: Contains chromogenic Streptococcus medium for isolating and differentiating Streptococcus:
          • agalactiae: Blue colonies
          • uberis: Blue-gray colonies
          • Enterococcus: Light to dark purple colonies
          • Other microorganisms are inhibited.
      Pack

      90mm three plates
      classify

      Three-compartment culture medium
      • Allow the culture plate to reach room temperature.
      • Dry the plate in an incubator by slightly opening the lid.
      • Inoculate the sample directly onto the medium surface for isolation.
      • Incubate aerobically at 35 – 37°C for 18 – 24 hours (extend if necessary).
      • Observe colony morphology and reactions (if any).

      RESULTS

      Observe growth characteristics such as colony morphology, color and size.

      • GNR Compartment
      Microorganism Colony Appearance
      E. coli Pink to dark red
      Klebsiella, Enterobacter, Citrobacter Metallic blue (+/- red halo)
      Proteus, Morganella, Providencia Brown halo
      Pseudomonas Transparent (+/- blue to green)
      Candida albicans Cream-colored, pointed colonies
      Gram (+) bacteria Inhibited
      • STAPH Compartment
      Microorganism Colony Appearance
      S. aureus Pink to lavender
      S. epidermidis Colorless to light pink
      S. saprophyticus Blue-gray
      Staphylococci khác Varying colors
      Streptococci Inhibited
      Gram (-) bacteria Inhibited
      • STREP Compartment
      Microorganism Colony Appearance
      S. agalactiae Blue
      S. uberis Blue to metallic blue
      Enterococcus Light to dark purple
      Other Streptococcus Varying colors
      Other Gram (+) bacteria Inhibited
      Gram (-) bacteria Inhibited

      BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

      Reference strains Incubation Conditions Expected results
      GNR Compartment    
      K. pneumoniae ATCC 13883  

       

      35 – 370C, 18 – 24 hours

      		 Good growth, blue-gray colonies
      Escherichia coli ATCC 25922 Good growth, light red colonies
      P. aeruginosa ATCC 10145 Good growth, transparent colonies
      S. aureus ATCC 25923 No growth, inhibited
      E. faecalis ATCC 29212 No growth, inhibited
      STAPH Compartment
      S. aureus ATCC 25923 35 – 370C, 18 – 24 hours Good growth, lavender colonies
      S. saprophyticus ATCC 15305 Good growth, turquoise colonies
      Escherichia coli ATCC 25922 No growth, inhibited
      C. albicans ATCC 10231 No growth, inhibited
      E. faecalis ATCC 29212 No growth, inhibited
      STREP Compartment
      S. agalactiae ATCC 13813 35 – 370C, 18 – 24 hours Good growth, blue colonies
      S. uberis ATCC 700407 Good growth, blue-gray colonies
      E. faecalis ATCC 29212 Good growth, purple colonies
      Escherichia coli ATCC 25922 No growth, inhibited
      C. albicans ATCC 10231 No growth, inhibited
      S. aureus ATCC 25923 No growth, inhibited
      • GNR Compartment (per liter, reference values)
      Agar 15,0 g
      Peptone and yeast extract 17,0 g
      Chromogenic mix 1,2 g

      pH of the ready-to-use medium at 250C: 7,4 ± 0,2

      • STAPH Compartment (per liter, reference values)
      Agar 15,0 g
      Peptone and yeast extract 40,0 g
      Salts 30,0 g
      Chromogenic and selective mix 2,4 g

      pH of the ready-to-use medium at 250C: 7,3 ± 0,2

      • STREP Compartment (per liter, reference values)
      Agar 15,0 g
      Peptones and growth factors 20,0 g
      Salts 7,5 g
      Chromogenic and selective mix 2,4 g
      Growth factors 8,0 mL

      pH of the ready-to-use medium at 25°C: 7,3 ± 0,2

      *Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.

      Product Name Code Packaging
      BacterPlate™ GNR/STAPH/STREP 3-compartment medium 05053 10 plates/ box or as per customer request.

      USER MANUAL_BacterPlate™ GNR/STAPH/STREP 3-compartment medium
      MSDS_BacterPlate™ GNR/STAPH/STREP 3-compartment medium
      COA_BacterPlate™ GNR/STAPH/STREP 3-compartment medium